The main aim of endodontic treatment is disinfection of root canal and to prevent chances of reinfection. The most commonly isolated species due to oral infections is Enterococcus faecalis. For nonsurgical endodontic procedures Sodium hypochlorite (NaOCl) has been the irrigant of choice. The mechanism by which endodontic irrigants induce cytotoxicity is still unclear. However, many studies clearly indicated that rapid expression of the reactive oxygen species (ROS) leads to free radicals formation which results in cytotoxicity and cell death. Hence this study was done to determine the viability of cells and oxidative stress mediated by NaOCl, an endodontic irrigant. The irrigants were tested for their effect against fibroblast isolated from human primary buccal mucosa and against 3T3 Cell line. Antibacterial activity was performed against Enterococcus faecalis. Cytotoxicity was determined by MTT. To determine the oxidative stress, total intracellular glutathione, superoxide radical scavenging activity, and catalase assays were performed. The MIC (Minimal Inhibitory Concentration) for the irrigants against Enterococcus faecalis was found to be 10 µl. 10 µl of NaOCl plain 5.2% produced the same effect as that of 10 μl of NaOCl plain 3%. The higher concentration of the irrigants decreased viability of the cells during dye exclusion assay. Enzyme based study showed there is a decrease in enzyme dehydrogenase when treat with irrigants. Glutathione, SOD level was increased gradually on 3T3 cells. But CAT level was increased when the irrigants concentration less. The results of this study indicated that endodontic irrigants were potentially controlling the Enterococcus faecalis and non-toxic/reduced viability of 3T3 cells by MTT which could be due to the oxidative stress and loss of cellular integrity probably due to the liberation of ROS evidenced by the alteration of antioxidant enzymes Glutathione, SOD and CAT.
Groups of Theileria annulata infected Hyalomma anatolicum anatolicum adults prefed on a calf for 1 to 6 days were separately ground in tissue culture medium-199, supplemented with bovine albumin powder, Fraction-V. Supernatant fluid was collected, made up with additional medium, so that each millilitre represented material from 25 ticks, and was injected subcutaneously into groups of cross-bred male calves. The results indicated that ground-up tick supernate (GUTS) prepared from unfed ticks was not infective, whereas that prepared from 1 to 6 days prefed ticks was infective. GUTS prepared from 3 days prefed ticks appeared to contain highest infectivity and 1 ml of it induced fatal theileriosis.
Aim:This study was conducted with the objective to evaluate the cytotoxicity of monomers isobutyl methacrylate (IBMA) and methacrylic acid (MA) in human buccal mucosal fibroblast primary cell culture and to study their effect on cellular enzymatic antioxidants-glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT).Materials and Methods:The tissue for fibroblast cell culture was harvested from oral buccal mucosa of a healthy donor. Fibroblast cells were plated at a density of 1 × 104 cells per well in 96-well tissue culture plates. Cells were exposed to various concentrations of IBMA and MA. The cell viability and various enzyme activities were evaluated 24 h after exposure to the above treatments. All tests were done in triplicate. Cell viability was assessed by trypan blue dye exclusion assay and all enzyme activities were done using assay kits from Cayman Chemicals, Ann Arbor, USA.Results:At all concentrations tested a statistically significant decrease in viability was observed in IBMA- and MA-treated cells. Around 42% cells were viable at the highest test concentration of IBMA (80 μmol/L) and only 20% cells were viable at the highest dose (144 μmol/L) of MA exposure (P < 0.05). Dose-dependent decrease in the GPx and SOD activities was observed in cells treated with IBMA and MA (P < 0.05). CAT activity was not detectable in the controls. However, a fall in CAT activity was detected in cells exposed to IBMA and MA at all concentrations tested (P < 0.05).Conclusion:IBMA and MA leaching out from the chairside denture hard reliners are cytotoxic on human buccal fibroblast primary cell cultures. This could be due to the oxidative stress caused by the generation of reactive oxygen species which is evidenced by the fall in activities of antioxidant enzymes (GPx, SOD, and CAT) and cytotoxicity.
The effects of rigid/non-rigid connectors and stress absorbing elements on mechanical behavior of TISP were studied using 2D finite element analysis. Finite element models were created in DISPLAY III software and were subjected to a static occlusal load of 75N. The use of non-rigid connector increased the stress (17.7 N/mm 2 ) on the implant neck when compared to rigid connector (10.25 N/mm 2 ) and stress absorbing element (8.49N/mm 2 ). Maximum displacement of the TISP (18.74 µm) was seen with a nonrigid connector.
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