PIEZO1 is a non-selective cation channel protein that converts mechanical stimuli into electrochemical signals through mechanical force transmission. In recent years, more and more attention has been paid to its relationship with cardiovascular development and related diseases. However, PIEZO1 is difficult to be used as a therapeutic target due to incomplete study of its related phenotype and mechanism. Starting with the phenotypes of zebrafish at different stages of development, piezo1 knockout zebrafish features decreased heart rate in embryonic stage, increased heart size in 72dpf larvae, as well as ventricular enlargement, passivation and increased immune infiltration in adult stage. Further characterization revealed the relationship between PIEZO1 and AcanA protein at embryonic stage, and hand2 protein at adult stage. Through further RNA-seq analysis, ptpn21 downregulation, shox2 upregulation, itga4 upregulation were used to explain ventricular enlargement, heart rate decrease and immune infiltration increase respectively. Overall, this study provides a theoretical basis for elucidating the mechanism of PIEZO1 in regulating cardiac development.
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