Highlightsd An ultrafast deconvolution tool for top-down mass spectrometry data is presented d A spectrum transformation that dramatically accelerates deconvolution is suggested d Our method reports more masses and substantially fewer artifacts than other tools

Abstrak -Telah dilakukan penelitian dan pengembangan media pembelajaran laboratorium virtual yang bertujuan untuk mengatasi miskonsepsi peserta didik materi fisika inti dan radioaktivitas Subjek uji coba adalah peserta didik kelas XII IPA SMA Binamu berjumlah 30 orang. Prosedur pengembangannya menggunakan model Four-D terdiri dari tahap pendefinisian, perancangan, dan pengembangan. Hasil penelitian menunjukkan (1) media laboratorium virtual model presentasi dan tutorial pada materi aktivitas zat radioaktif dan daya tembus sinar radioaktif diperoleh hasil valid dan reliabel. Setiap tampilan dilengkapi navigasi, petunjuk, hyperlink,dan fasilitas lainnya untuk memudahkan penggunaan program, (2) perangkat pembelajaran berupa Rencana Pelaksanaan Pembelajaran, Buku Bacaan, dan Lembar Kerja Peserta Didik selain dibuat dalam bentuk hardcopy, juga softcopy autorun CD. Penilaian menunjukkan valid dan reliabel, (3) aktivitas peserta didik di atas 85%, menunjukkan pembelajaran yang dilakukan mampu mengaktifkan peserta didik. Persentase persepsi peserta didik adalah 93,05% menunjukkan sangat setuju terhadap pembelajaran fisika berbasis media laboratorium virtual, (4) berdasarkan tes akhir, terjadi peningkatan pemahaman konsep yang baik peserta didik dibandingkan sebelum diberi media laboratorium virtual. Abstract -The develovment research of learning instrument of virtual laboratory was conducted which aimed at contending themisconception about nuclear physics. The subject of the study was 30 students grade XII IPA at SMA 1 Binamu,Jeneponto.The procedure of development referred to four-D model consisted of definition phase, design phase, and development phase. The result of the study revealed that (1) the virtual laboratory media developed in form of presentation and tutorial model that consisted of radioactivity-decay and emerge power of radioactive radiation gotten valid and reliable result.each of media display completed with navigation, program instruction, hyperlink and several other facilities to allow users to run the program.(2)the learning instrument in form of lesson plan, the textbook, and student's workbook presented in softcopy of CD autorun.product was valid and reliable. (3) the student's activities during the learning process were above 85%, show that the students agree with instrument physiscs learning basis on virtual laboratory. (4) according to the tes result, the concept comprehension of students was improve than before given virtual laboratory media.

Feature deconvolution, the determination of intact proteoform masses, is crucial for top-down proteomics, but currently suffers from long runtimes and quality issues. We present FLASHDeconv, an algorithm based on a simple transformation of mass spectra, which turns deconvolution into the search for constant patterns thus greatly accelerating the process. We show higher deconvolution quality and two to three orders of magnitude faster execution speed than existing approaches.

The demand for reliable comparability studies of biosimilars grows with their increased market share. These studies focus on physicochemical, structural, functional and clinical properties to ensure that a biosimilar has no significant differences to the originator product and can be released into the market without extensive clinical trials. In the current study, Enbrel® (etanercept, the originator) and Altebrel™ (the proposed biosimilar) underwent direct comparison. “Bottom-up” mass spectrometric analysis was used for primary sequence analysis, evaluation of N/O-glycosylation sites and quantification of methionine oxidation. N/O-glycans were analyzed after permethylation derivatization and the effect of N-glycans on in-vitro functionality of etanercept was assayed. Three enzyme peptide mapping resulted in complete identification of the primary structure. It was confirmed that total ion chromatograms are valuable datasets for the analysis of the primary structure of biodrugs. New N/O-glycan structures were identified and all the N-glycans were quantified. Finally, investigation of the functional properties of N-deglycosylated and non-modified etanercept samples using surface plasmon resonance analysis and in-vitro bioassay showed that N-glycosylation has no significant effect on its in-vitro functionality. Analysis of etanercept and its biosimilar, revealed a high similarity in terms of glycosylation, primary structure and in-vitro functionality.

No abstract

Penelitian ini bertujuan untuk memberikan solusi dan informasi kepada dunia pendidikan mengenai model pembelajaran yang efektif dan interaktif dalam menyongsong masa revolusi industri 4.0. Fokus penelitian ini adalah dengan adanya model pembelajaran berbasis hybrid learning. Model pembelajaran yang menggabungkan atau menyatukan antara pembelajaran secara  online dengan pembelajaran secara bertatap muka dengan peserta didik, karena dalam masa revolusi industri 4.0 ditandai dengan kemajuan teknologi informasi sebagai media utama dalam kehidupannya. Metode dalam penelitian ini yaitu dengan menggunakan pendekatan kualitatif deskriptif dengan teknik pengambilan data melalui studi pustaka. Hasil penelitian ini menunjukkan bahwa model pembelajaran berbasis Hybrid Learning  efektif digunakan dalam pengajaran di kelas pada dunia pendidikan di masa revolusi 4.0 ini, karena metode ini adalah metode yang menggabungkan 2 cara yaitu pengajaran dengan online dan tatap muka, yang mana dalam era revolusi industri 4.0 lebih mengedepankan teknologi atau era big data. Maka dari itu guru maupun dosen perlu berinovasi dalam menyongsong masa revolusi industri 4.0 dengan model pembelajaran yang efektif dan interaktif seperti Hybrid Learning.

This study aimed to observe image construction of the Islamic Defenders Front (FPI) based on six articles of the Jakarta Post Online News about the FPI’s protest toward three issues: Ahmadiyah, the arrival of Lady Gaga in Indonesia, and Ahok as the substitute governor of Jakarta. The author qualitatively explored the FPI’s image using Halliday’s systemic functional linguistics (SFL), which defines the types of process in a clause. The author also analyzed the thematic structure of the clause to support the analysis of each type of process. The result was approximately 208 clauses from the six news articles. This study shows that the dominant process in the above-mentioned three issues is the material process. The material process represents 51% in the issue of Ahmadiyah, 41% in the issue of the arrival of Lady Gaga, and 70% in the issue of Ahok as Jakarta’s substitute governor. This material process shows that the Jakarta Post Online News constructed a negative image of the FPI’s by reporting this organization’s action during the protest. In the issue of Ahmadiyah, the FPI was framed as anarchist, while in the issue of Ahok and of Lady Gaga it was represented as protestor. The analysis of thematic structures shows the FPI as the dominant topical theme, which indicates the FPI was the main topic of the articles.

Purpose of this research is to find out: (1) What is the general picture of Yogyakarta during the Physical Revolution in Indonesia (2) What is the General Picture of Ulama during the Physical Revolution in Yogyakarta (3) How the Role of Muhammadiyah Ulama in the Formation of APS (Askar War Sabil) in Yogyakarta. The method used in this research is Literature with five stages, namely: (1) Selection of topics, determining the main points of the problem to be studied, (2) Heuristics, (3) Criticizing sources, (4) interpretation, analyzing sources that have been tested for their validity, (5) histiography, chronological historical arrangement so that historical writing is compiled. The results of this study indicate (1) the Physical Revolution in Yogyakarta occurred due to several things, namely, the Dutch Military Aggression I, the PKI Rebellion in Gunungkidul, the Dutch Military Aggression II and the March 1 General Offensive, (2) During the Physical Revolution in Yogyakarta, scholars such as NU, MIAI have a role in fighting for independence, one of which is in establishing Islamic warriors such as Hisbullah and Sabilillah (3) the ulama in Yogyakarta, namely Muhammadiyah also participated in the Physical Revolution, namely in the formation of APS and in providing Physical, Idiological and Physical exercises. Spirituality.