Caffeine is naturally found in plant products such as coffee beans, cocoa beans and tea leaves. In this study, caffeine molecular imprinted polymer (MIP) was synthesized by dissolving 1 mmol caffeine and 4 mmol monomer, methacrylic acid (MAA) and 1mmol ethylene glycol dimethacrylate (EDMA) as the crosslinker and benzoyl peroxide (BOP) as the initiator. MIP exhibits significantly high affinity and selectivity for the target analytes used as the template than for similar molecules, including closely related isomers. The efficiency of extraction using caffeine MIP as sorbents in solid phase extraction (SPE) was evaluated. Good recovery (91.6 %) and low percent RSD (0.9 %) was obtained using the spiked samples (10 µg/mL). The molecular imprinted polymer solid phase extraction (MISPE) technique was then applied in the extraction of caffeine from various coffee samples. Based on the excellent selectivity, MIP is a promising approach for the extraction of compounds from a complex matrix such as herbal extract.
Caffeine is naturally found in plant products such as coffee beans, cocoa beans and tea leaves. In this study, caffeine MIP was synthesized by dissolving 1 mmol caffeine and 4 mmol monomer, methacrylic acid (MAA) and 1mmol ethylene glycol dimethacrylate (EDMA) as the crosslinker and benzoyl peroxide (BOP) as the initiator. Molecular imprinted polymers (MIP) exhibits significantly high affinity and selectivity for the target analytes used as the template than for similar molecules, including closely related isomers. The efficiency of extraction using caffeine MIP as sorbents in solid phase extraction (SPE) was evaluated. Good recovery (91.6 %) and low percent RSD (0.9 %) was obtained. The MISPE technique was applied in the extraction of caffeine from various coffee samples. Based on the excellent selectivity, MIP is a promising approach for the extraction of compounds from a complex matrix such as herbal extract.
In this study, three parameters (sample volume, cleanup and elution step) in large volume injection online Solid Phase Extraction Liquid Chromatography with diode array detection (SPE-LC) were optimized for the determination of six pharmaceuticals in wastewater samples. Preconcentration and separation were carried out using 2 columns, Dionex Ion Pac AG14A and Acclaim Polar Advantage II. Response Surface Methodology (RSM) was employed for the optimization of cleanup step (mobile phase composition and valve switching time). The optimum conditions obtained were as follows: Sample volume of 10.0 mL and cleanup with acetonitrile and ultrapure water (5:95) for 1.0 min. The method showed good recovery (82.91-107.7%), precision (0.35-8.26%) and linearity (R 2 >0.99). The limit of detection was between 3.5 and 11.7 µg L −1 . The proposed method is rapid, simple and sensitive for trace analysis of pharmaceuticals in water samples.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.