Carotenoid esterification plays indispensable roles in preventing degradation and maintaining the stability of carotenoids. Although the carotenoid biosynthetic pathway has been well characterized, the molecular mechanisms underlying carotenoid esterification, especially in floral organs, remain poorly understood. In this study, we identified a natural mutant flowering Chinese cabbage (Caixin, Brassica rapa L. subsp. chinensis var. parachinensis) with visually distinguishable pale-yellow petals controlled by a single recessive gene. Transmission electron microscopy (TEM) demonstrated that the chromoplasts in the yellow petals were surrounded by more fully developed plastoglobules compared to the pale-yellow mutant. Carotenoid analyses further revealed that, compared to the pale-yellow petals, the yellow petals contained high levels of esterified carotenoids, including lutein caprate, violaxanthin dilaurate, violaxanthin-myristate-laurate, 5,6epoxy-luttein dilaurate, lutein dilaurate, and lutein laurate. Based on bulked segregation analysis and fine mapping, we subsequently identified the critical role of a phytyl ester synthase 2 protein (PALE YELLOW PETAL, BrPYP) in regulating carotenoid pigmentation in flowering Chinese cabbage petals. Compared to the yellow wild-type, a 1,148 bp deletion was identified in the promoter region of BrPYP in the pale-yellow mutant, resulting in down-regulated expression. Transgenic Arabidopsis plants harboring beta-glucuronidase (GUS) driven by yellow (BrPYPY::GUS) and pale-yellow type (BrPYPPY::GUS) promoters were subsequently constructed, revealing stronger expression of BrPYPY::GUS both in the leaves and petals. Furthermore, virus-induced gene silencing of BrPYP significantly altered petal color from yellow to pale yellow. These findings demonstrate the molecular mechanism of carotenoid esterification, suggesting a role of phytyl ester synthase in carotenoid biosynthesis of flowering Chinese cabbage.
The launch of the Haiyang-2 (HY-2) satellite constellation fosters the quick acquisition of global sea surface vector winds from the perspective of remote sensing. This study intends to develop a six-hourly mesoscale analysis of sea surface winds based on the microwave scatterometers onboard the HY-2 satellite series, with the objective of meeting the considerable demand for accurate and gap-free ocean wind forcing products. First, the accuracy of HY-2 scatterometers (HSCATs) in measuring wind is evaluated. In particular, the standard deviation (SD) errors of HSCATs data are assessed using the collocated buoy measurements with different temporal windows in order to account for the temporal representativeness errors in the blending analysis. Afterwards, a two-dimensional variational (2D-Var) method is implemented to blend the HSCATs measured winds and the European Centre for Medium-Range Weather Forecasts (ECMWF) reanalysis winds over the global ocean surface. This approach is different from existing methods in that it takes both wind error and background error correlation into account. The results show that the blended wind product is of a promising quality compared with independent wind references. Interestingly, the blended winds derived from 2D-Var in combination with an empirical background error correlation show smaller SD errors than those using a Gaussian error correlation function. Overall, the blended wind product should be valuable for forcing global ocean models or describing air-sea interaction processes on a scale close to the scatterometer measurements.
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