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The effect of dietary n-3 series fatty acids on the number of pleopadal egg and stage 1 juvenile in freshwater crayfish, Astacus leptodactylus was investigated. Crayfish were fed with n-3 series fatty acids supplemented diets and a control diet for 251 days. Control diet did not comprise additional n-3 series fatty acids. However, D2, D3 and D4 groups were supplemented with n-3 series fatty acids at 1%, 2% and 3% level respectively. Results showed the beneficial effects of dietary n-3 series fatty acids on the production of pleopodal egg and stage 1 juvenile number. At the end of the experiment, pleopodal egg (from 177 to 234) and stage 1 juvenile number (from 167 to 225) increased significantly with increased dietary n-3 series fatty acid level. In addition, an increase in dietary n-3 series fatty acids led to an increase in number of pleopodal egg and stage 1 juvenile produced per gram of female crayfish weight. It can be concluded that the reproductive efficiency of A. leptodactylus (i.e., pleopodal egg and stage 1 juvenile number) can be improved in controlled hatchery conditions by adding n-3 series fatty acid into the diet of this species.

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In this study, the testis and semen fatty acid, fat-soluble vitamin and sterol levels, testis-somatic index and spermatological values were investigated in the broodstock rainbow trout fed with the different levels of omega-3 polyunsaturated fatty acid (PUFA) under the regular stripping conditions. For this purpose, a control and three test diets were prepared. The control, D1, D2 and D3 diets were contained the omega-3 PUFA at 3.14%, 7.84%, 13.63% and 20.54% as a percentage of the total fatty acids respectively.The fish in the experimental groups were fed with these diets for 60 days. The testis-somatic index (3.81-4.50), semen volume (9.30-18.5 ml) and pH (8.05-8.99), initial sperm motility (68.1%-93.7%), duration of 50% sperm motility (141.3-258.9 s), total duration of sperm motility (268.7-489.6 s) and sperm density (9.95-17.5 cells × 10 9 ml −1 ) values of the fish fed the control diet were significantly decrease (p < 0.05) depending on decline of the omega-3 PUFA, fat-soluble vitamin and sterol contents in the testis and semen. The omega-3 PUFA (3.40%-14.76% and 3.96%-16.31%), vitamin A (33.9-61.2 ml/mg and 9.12-26.7 ml/mg), D 2 (0.10-0.19 ml/mg and 0.04-0.10 ml/mg), D 3 (0.75-1.30 ml/mg and 0.06-0.24 ml/mg), alpha-tocopherol (30.1-60.4 ml/mg and 8.93-25.3 ml/mg), K 1 (0.30-0.94 ml/mg and 0.03-0.08 ml/mg) and K 2 (2.0-3.19 ml/ mg and 0.25-0.34 ml/mg) vitamins, cholesterol (1,201-1,588 ml/mg and 330-596 ml/ mg), ergosterol (3.12-6.40 ml/mg and 2.10-4.08 ml/mg), stigmasterol (5.57-10.4 ml/ mg and 10.2-12.6 ml/mg) and beta-sitosterol (0.20-0.60 ml/mg and 0.14-0.41 ml/mg) contents in the testis and semen of the fish were significantly affected (p < 0.05) by the dietary omega-3 PUFA supplementation. The highest values for these parameters were provided from the fish fed the D2 and D3 diets (p > 0.05), followed by the fish fed the D1 diet (p < 0.05). These results showed that the dietary omega-3 PUFA at 13.63% of the total fatty acids could increase the omega-3 PUFA, fat-soluble vitamin and sterol contents in the testis and semen, the testis-somatic index and spermatologic values of the broodstock rainbow trout under the regular stripping conditions. K E Y W O R D Sfat-soluble vitamins, fatty acids, rainbow trout, sperm, sterols, testis

In this study, the semen production and quality, hepato‐somatic index, haemato‐immunologic values, oxidative stress and the fatty acid contents in liver, muscle and semen of rainbow trout fed diets supplemented n‐3 series long‐chain polyunsaturated fatty acids (LC‐PUFAs) under regular stripping condition were investigated. For this aim, three diets (Control, D1 and D2) were prepared. These diets were contained n‐3 LC‐PUFAs (as a percentage of dietary total fatty acid) at 3.14%, 7.84% and 13.63% respectively. Experimental fish were fed with the control and test diets. The highest hepato‐somatic index, spermatologic (semen pH and volume, sperm motility and density), haematologic (haematocrit value, haemoglobin, erythrocyte count, corpuscular volume, haemoglobin and its concentration in corpuscular), immunologic (nitroblue tetrazolium activity, leucocyte count, phagocytic index, protein and immunoglobulin [IgM] in total plasma) and antioxidants (reduced glutathione, catalase, glutathione peroxidase and superoxide dismutase) values were found in fish fed the D2, D1 and control diets respectively (p < 0.01). Increase in the dietary n‐3 LC‐PUFAs was not significantly (p > 0.01) increased the oxidative stress (malondialdehyde) in fish. The results indicated that the n‐3 LC‐PUFAs at 13.63% level of total fatty acid in the diet could increase the semen production and quality, hepato‐somatic index, haematologic and immunologic values, and the n‐3 LC‐PUFA contents in liver, muscle and semen of rainbow trout broodstock under regular stripping condition.

Objective: This study was performed to determine the effect of different ratios of n-3 series fatty acids added to the diets of rainbow trout (Oncorhynchus mykiss) on sperm motility duration.Methods: A total of 48 male rainbow trouts about 3 years of age were used in the study and 4 groups were formed. The one that n-3 series fatty acids were not added to the diet was used as control group. n-3 series fatty acids were added to the experimental groups diets at the ratios of 1% (E1), 2% (E2) and 3% (E3), respectively. Semen was collected by abdominal massage. The time-dependent sperm motility change was detected under the microscope at x400 magnification.Results: A significant decrease was detected in the motility only at 360th hour in E3 group compared to the control group (p<0.05). Conclusion:As a result, it was concluded that feeding with diets containing different ratios of n-3 series fatty acids had no effect on the motility of rainbow trout semen under in vitro condition.