BackgroundClinical and experimental data suggest that the inflammatory response is impaired in diabetics and can be modulated by insulin. The present study was undertaken to investigate the role of insulin on the early phase of allergic airway inflammation.MethodsDiabetic male Wistar rats (alloxan, 42 mg/Kg, i.v., 10 days) and controls were sensitized by s.c. injection of ovalbumin (OA) in aluminium hydroxide 14 days before OA (1 mg/0.4 mL) or saline intratracheal challenge. The following analyses were performed 6 hours thereafter: a) quantification of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 in the bronchoalveolar lavage fluid (BALF) by Enzyme-Linked Immunosorbent Assay, b) expression of E- and P- selectins on lung vessels by immunohistochemistry, and c) inflammatory cell infiltration into the airways and lung parenchyma. NPH insulin (4 IU, s.c.) was given i.v. 2 hours before antigen challenge.ResultsDiabetic rats exhibited significant reduction in the BALF concentrations of IL-1β (30%) and TNF-α (45%), and in the lung expression of P-selectin (30%) compared to non-diabetic animals. This was accompanied by reduced number of neutrophils into the airways and around bronchi and blood vessels. There were no differences in the CINC-1 levels in BALF, and E-selectin expression. Treatment of diabetic rats with NPH insulin, 2 hours before antigen challenge, restored the reduced levels of IL-1β, TNF-α and P-selectin, and neutrophil migration.ConclusionData presented suggest that insulin modulates the production/release of TNF-α and IL-1β, the expression of P- and E-selectin, and the associated neutrophil migration into the lungs during the early phase of the allergic inflammatory reaction.
O presente trabalho teve como tema o impacto do envelhecimento em mulheres a partir de 60 anos,sob a esfera gerontológica a qual nos encontramos, em que o estereótipo de beleza tem sidobastante difundido, os idosos nos trazem um questionamento de como é encarada sua percepçãopessoal de envelhecimento nos tempos atuais. Este trabalho objetivou abordar as questõespertinentes ao processo de envelhecimento em mulheres, onde nos deparamos com uma crescentepopulação de idosos. A metodologia aplicada foi a abordagem quali-quantitativa com mulheresem faixa etária igual ou superior a 60 anos. Aborda-se a respeito da percepção do envelhecimentoe conclui-se que a grande maioria se encontra satisfeita com sua autoimagem. Possivelmente,morar com familiares deve ser um fator que agrega sua participação no vínculo social e cuidadoscom a autoestima
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