Inflammation accompany pregnancy for physiological reasons but can be
also involved in pathological process with deleterious effect on
placental function and fetal development. Transmembrane exchange of
essential and waste substances in the placenta is one of the most
important placental roles. This study focusses on two solute carrier
transporters, OCTN1 (SLC22A4) and OCTN2 (SLC22A5), with OCTN2 playing a
role in the placental uptake of L-carnitine and OCTN1 generally believed
to ensure cell protection against oxidative stress. We employed two
cellular models and ex vivo cultured human placental villous explants to
address possible changes in the expression of placental OCTN1/2 upon
inflammatory environment. Our data reveal the upregulation of OCTN1/
SLC22A4 in HTR-8/SVneo treated with TNF-α and IFN-γ. LPS was able to
enhance OCTN1 expression in explants and IFN γ increased expression of
OCTN1 in BeWo cells. OCTN2/ SLC22A5 expression was upregulated upon
exposition of IFN-γ in HTR-8/SVneo cells but decreased following
treatment of BeWo cells with TNF-α. TNF-α and also LPS further decreased
expression of OCTN2 in ex vivo explants. In conclusion, our data show
for the first time that the gene expression of OCTN1 as well as OCTN2
can be affected by inflammatory environment in human placenta.
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