Supplementary data are available at Bioinformatics online.

The SIB Swiss Institute of Bioinformatics (www.isb-sib.ch) provides world-class bioinformatics databases, software tools, services and training to the international life science community in academia and industry. These solutions allow life scientists to turn the exponentially growing amount of data into knowledge. Here, we provide an overview of SIB's resources and competence areas, with a strong focus on curated databases and SIB's most popular and widely used resources. In particular, SIB's Bioinformatics resource portal ExPASy features over 150 resources, including UniProtKB/Swiss-Prot, ENZYME, PROSITE, neXtProt, STRING, UniCarbKB, SugarBindDB, SwissRegulon, EPD, arrayMap, Bgee, SWISS-MODEL Repository, OMA, OrthoDB and other databases, which are briefly described in this article.

The popularity of rural areas and especially the mountainous ones, as a possibility to spend the vacation has increased in most countries. Almost all mountain areas can provide great opportunities for the development of various forms of rural tourism, there being many examples according to which many mountain areas, through appropriate and effective strategies of capitalizing the resources through the forms of rural tourism, start from the pioneer stage to a real valuable alternative in socio-economic terms for the rural area. One of the concepts that are increasingly used is the "tourist village", its main issue, consisting in the return of the investment, an aspect that we will discuss in this paper. The paper presents an analysis of the way in which the occupancy degree affects the period of amortization of the initial investment. Concretely, in the case of the medium-sized tourist village, the minimum occupancy degree for the amortization of the initial investment, starts from about 25% for the investment without a loan, but subsidy in proportion of 50% and reaches at about 85% in the case of an investment with loan, for a period of 10 years, without advance or subsidy and with an interest of 7%. Initial investments were established for a holiday village in the Romanian countryside.Sustainability 2017, 9, 875 2 of 18 required by the city dwellers and tourists; travel of 30-50 km from the cities become competitive with long-distance journeys abroad; and most city dwellers prefer to spend leisure and weekend days in a quiet, green and clean environment.Rural tourism can be a source of income for the rural population. The traditional products and resources that can be capitalized through this form of tourism are: traditional architecture, traditional objects, folk costumes, folk sculpture, traditional folk instruments, folklore manifestations, various traditional gastronomic, and eco-friendly products; in other words, the specific rural way of life, with multiple benefits for all participants to this activity. In order for rural tourism to have a high value, it is necessary that the economic value of adjacent activities to take place at a high level, so for this reason a rural tourist village could help.The aim of the paper. The idea of this paper starts from the existence of numerous locations with tourism potential. Many of these locations can be found in the countryside, so the idea of rural tourism is brought to the fore. In other words, the aim is to combine local economic interests, the development of communities, with those aiming at the conservation of tourism resources. The motivation for choosing a tourist village is that it could be the center of generating profit when it "sells" rooms at a higher price than operating costs. The purpose of the paper is to highlight and study the concrete situation regarding the investments in developing a holiday village that contains 20 rooms, possibly located in Apuseni Mountains. The research objective is a tourist village built from scratch, so the purpose of the ...

In contrast to urodele amphibians and teleost fish, mammals lack the regenerative responses to replace large body parts. Amphibian and fish regeneration uses dedifferentiation, i.e., reversal of differentiated state, as a means to produce progenitor cells to eventually replace damaged tissues. Therefore, induced activation of dedifferentiation responses in mammalian tissues holds an immense promise for regenerative medicine. Here we demonstrate that ectopic expression of Msx2 in cultured mouse myotubes recapitulates several aspects of amphibian muscle dedifferentiation. We found that MSX2, but not MSX1, leads to cellularization of myotubes and downregulates the expression of myotube markers, such as MHC, MRF4 and myogenin. RNA sequencing of myotubes ectopically expressing Msx2 showed downregulation of over 500 myotube-enriched transcripts and upregulation of over 300 myoblast-enriched transcripts. MSX2 selectively downregulated expression of Ptgs2 and Ptger4, two members of the prostaglandin pathway with important roles in myoblast fusion during muscle differentiation. Ectopic expression of Msx2, as well as Msx1, induced partial cell cycle re-entry of myotubes by upregulating CyclinD1 expression but failed to initiate S-phase. Finally, MSX2-induced dedifferentiation in mouse myotubes could be recapitulated by a pharmacological treatment with trichostatin A (TSA), bone morphogenetic protein 4 (BMP4) and fibroblast growth factor 1 (FGF1). Together, these observations indicate that MSX2 is a major driver of dedifferentiation in mammalian muscle cells.