S i h a m B a yo u mi S a l e m Do n a l d B . Mi l e s A b e e r A b d -E l h a me e d A l m -E l d e e n Ra m a d a n M a h m o u d K a n d ye lComparative histological characterization of the tongue in two reptiles, Scincus scincus (Scincidae) and Uromastyx ornata (Agamidae) in relation to diet
ABSTRACT:The present study is designed to investigate the morphological and histological characterization of the tongue in two reptilian species; S. scincus (Insectivorous) and U. ornata (Herbivorous). Moreover, to reveal the histochemical features of the lingual glands and connective tissue fibres. Five adult reptiles of each species were used, they were collected from Abo-Rawash, Egypt. Different histochemical methods were used to detect the nature of the lingual gland secretions and their relationship with the feeding habits. Morphologically, in both species, the tongue is somewhat triangle with three distinguishable areas; apex, body, and root. The notched tip is more obvious in U. ornata. Different shapes of papillae are distributed over the dorsal lingual surfaces and being more abundant in U. ornata. Both serous and mucous secretory acini are noticed in the tongue of U. ornate, however, in S. scincus only mucous acini are found. The secretory acini showed a clear affinity toward all stain types according to the acid and neutral mucin. The amount and distribution of collagen fibres are comparatively more in the tongue of U. ornata. Elastic fibres are not detected in the two-examined species. The results showed a marked variation in lingual papi llae, lingual gland secretions, and in the distribution of collagen fibres in the two -examined species as a reflection of their diet.
KEY WORDS:Lingual papillae, Lingual glands, Taste buds, Collagen fibres, Herbivorous
CORRESPONDENCE:S i h a m B a yo u mi S a l e m Zoology Department, Faculty of Science, Tanta University, Egypt.
Zinc oxide nanoparticles (ZnO NPs) are involved in different applications such as nutrition, while, it causes damage to different organs of the body including the brain. This study aimed to compare between the effect of ZnO NPs on newborn and adult rats´ cerebellum and to evaluate the protective role of Ethylenediaminetetraacetic acid (EDTA) against ZnO NPs toxicity. Thirty-two newborn (NBM)and thirty-two adult male (ADM) Sprague Dawley rats are used. Four groups (n = 8) of each are divided as following; Gp1: is served as control, Gp2: injected intraperitoneal (IP) with ZnO NPs (3 g/ kg). Gp3: injected IP with EDTA (500 mg/ kg) for 7 days. Gp4: injected with ZnO NPs and treated with EDTA as in Gp3. Brain tissue is prepared for biochemical and histopathological investigations. Newborn and adult rats injected with ZnO NPs showed degeneration of purkinje and granular cells in cerebellum tissue confirmed with high expression of caspase-3, while, treatment with EDTA post ZnO NPs showed lightly improvement. The body weights and relative brain weights is decrease in all the rats treated with ZnO NPs. In addition, our current study proved that the effect of ZnO NPs on ADM rats is more than on the NBM ones. Collectively, ZnO NPs injection caused damage of the cerebellum in newborn and adult rats, however, the treatment with EDTA stopped somewhat this damage.
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