Mycobacterium tuberculosis is the causative agent of the
disease, tuberculosis and H37Rv is the most studied clinical strain. We use
comparative genome analysis of Mycobacterium tuberculosis H37Rv
and human for the identification of potential targets dataset. We used DEG
(Database of Essential Genes) to identify essential genes in the H37Rv strain.
The analysis shows that 628 of the 3989 genes in Mycobacterium
tuberculosis H37Rv were found to be essential of which 324 genes
lack similarity to the human genome. Subsequently hypothetical proteins were
removed through manual curation. This further resulted in a dataset of 135
proteins with essential function and no homology to human.
A thermophilic fungal strain producing pectinases was isolated afterprimary screening of 12 different isolates on modified Pectin agar medium usingdifferent compost and decomposed matter collected from the vegetable and fruitmarkets of different cities of U.P. Selection of the fungus was done based on theclearance zones and pectinase enzyme production carried out in solid statefermentation. The fungal isolate was initially identified as a Mucor sp.
Present investigation deals with the isolation and screening of Aspergillus sp. forthe biosynthesis of xylanase. Isolation of fungi was carried out on Potato Dextrose Agar(PDA) followed by serial dilution method using the samples obtained from the soil collectedfrom different areas of Meerut. The culture was incubated at 30 0C for 3-5 days. The fungalisolates were subculture to purity and examine for xylanolytic activities. Screening forxylanolytic activities was also performed on potato dextrose agar (PDA) containing 0.1%(w/v) of xylan from oat spelt. Positive xylanolytic isolates were detected based on the clearzones of hydrolysis on the xylan. The fungi were considered forxylanase production.
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