MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 siRNA can accelerate mitotic exit. MCM7 depletion leads to CDK1 inactivation and promotes subsequent cohesin/RAD21 cleavage, which eventually leads to sister chromatin segregation. Moreover, MCM7 is co-localized with tubulin in the mitotic cells and MCM7 depletion results in aberrant mitosis. Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity.
UNC93B1 is a trafficking chaperone of endosomal Toll‐like receptors (TLRs) and plays an essential role in the TLR‐mediated innate signaling. However, whether it is also involved in other innate immune sensing or cellular pathways remains largely unexplored. Here we investigated the role of UNC93B1 in cytosolic DNA‐triggered cGAS‐STING signaling in mouse and human cell lines. We showed that while UNC93B1 deficiency blunts the signal transduction by TLR3, it augments innate immune responses to cytosolic DNA stimulation and DNA virus infection. Mechanistic study reveals a distinct action of UNC93B1 upon STING, but not other parts along the cGAS‐STING‐TBK1 axis, through regulating the protein level of STING at both resting and cytosolic DNA‐stimulated conditions. UNC93B1 can directly interact and traffic along with STING, and the disruption of this interaction causes accumulation of STING that subsequently leads to augmented signaling responses upon its activation. These findings reveal a new function of UNC93B1 in negatively regulating STING‐mediated signaling responses.
The buckle before the lens coating is still typically disassembled manually. The difference between the buckle and the background is small, while that between the buckles is large. This mechanical disassembly can also damage the lens. Therefore, it is important to estimate pose with high accuracy. This paper proposes a buckle pose estimation method based on a generative adversarial network. An edge extraction model is designed based on a segmentation network as the generator. Spatial attention is added to the discriminator to help it better distinguish between generated and real graphs. The generator thus generates delicate external contours and center edge lines with help from the discriminator. The external rectangle and the least square methods are used to determine the center position and deflection angle of the buckle, respectively. The center point and angle accuracies of the test datasets are 99.5% and 99.3%, respectively. The pixel error of the center point distance and the absolute error of the angle to the horizontal line are within 7.36 pixels and 1.98°, respectively. This method achieves the highest center point and angle accuracies compared to Hed, RCF, DexiNed, and PidiNet. It can meet practical requirements and boost the production efficiency of lens coatings.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.