The indirect electroreductive coupling of aryl halides and pyrroles was successfully conducted using a catalytic amount of perylene bisimide as a mediator in 1-ethyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)imide ([EMIM]NTf2)/DMSO.
Understanding the biosynthetic pathways of fungal pigments
can
help elucidate their roles in fungal growth processes. Trichodimerol
is a unique cage-like dimeric sorbicillinoids pigment that is commonly
isolated from many fungi, however, its biosynthesis is just partially
clarified. In this study, we report that a biosynthetic gene cluster
encoded major facilitator superfamily transporter (StaE) from the
fungus Stagonospora sp. SYSU-MS7888
is involved in the formation of trichodimerol, together with several
other dimeric sorbicillinoids. Using Aspergillus oryzae NSARI as a heterologous host, we demonstrated that the formation
of dimeric sorbicillinoids required co-expression of the transporter
StaE with biosynthetic genes (two PKSs and one monooxygenase) that
are responsible for constructing the monomer precursor sorbicillinol.
Fluorescence microscopy results showed that eGFP-tagged StaE is localized
on the endoplasmic reticulum, suggesting that sorbicillinoid dimerizations
might be compartmentalized in this organelle.
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