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ABSTRACIA new indole-3-acetic acid metabolite was isolated from broad bean (Viciafaba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnetic resonance, and mass spectra. Its natural content in 4-day-old Vicia seedlings was estimated to be 8.6 nanomoles per gram fresh weight. It was suggested that oxidation of indole-3-acetic acid not accompanied by decarboxylation might regulate endogenous level of the hormone.Exogenously supplied IAA is known to be metabolized to indole-3-aldehyde, indole-3-acetylaspartic acid and l-(indole-3-acetyl)-f,-glucose in various plant species (3, 7, 24, 31), esterified to IAA-myo-inositol in Zea seeds-(7), and conjugated with various amino acids in crown gall tissue (8). Ueda

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