Recently, the fluorescent detection of carbon monoxide (CO) in living cells has attracted great attention. However, due to the lack of effective ways to construct fluorescent CO probes, fluorescent detection of CO in living cells is still in its infancy. In this paper, we report for the first time the use of allyl ether as a reaction site for construction of fluorescent CO probes. By this way, two readily available allyl fluorescein ethers were prepared, which were found to be highly selective and sensitive probes for CO in the presence of PdCl. These probes have the merits of good stability, good water-solubility, and rapid and distinct colorimetric and remarkable fluorescent turn-on signal changes. Moreover, a very low dose of these two probes can be used to detect and track CO in living cells, indicating that these two probes could be very promising biological tools for CO detection in living systems. Overall, this work provided not only two new promising fluorescent CO probes but also a new way to devise fluorescent CO probes.
Carbon monoxide (CO) is an important gasotransmitter in living systems and its fluorescent detection is of particular interest. However, fluorescent detection of CO in living cells is still challenging due to lack of effective probes. In this paper, a readily available fluorescein-based fluorescent probe was developed for rapid detection of CO. This probe can be used to detect CO in almost wholly aqueous solution under mild conditions and shows high selectivity and sensitivity for CO with colorimetric and remarkable fluorescent turn-on signal changes. The detection limit of this probe for CO is as low as 37 nM with a linear range of 0-30 μM. More importantly, this probe (1 μM dose) can be conveniently used for fluorescent imaging CO in living cells.
A visible-light-triggered metal-free CO-releasing molecule with distinct ratiometric fluorescence changes was reported for CO release in living systems.
To
elucidate the complex role of biological H2S and
study the mitochondrial damage and some related diseases, effective
methods for visualization of H2S in mitochondria and in
vivo are urgently needed. In this contribution, a novel near-infrared
mitochondria-targetable fluorescence probe MI-H
2
S for H2S detection was developed. MI-H
2
S shows rapid detection
ability for H2S in pure aqueous solution and outputs a
highly selective and sensitive fluorescence-on signal at 663 nm with
a large Stokes shift of 141 nm. Bioimaging experiments revealed that
the probe has good mitochondrial-targeting ability and high-contrast
imaging ability for detecting H2S in living systems. The
probe also showed great potential in the detection of H2S during inflammation. All of the results demonstrate that MI-H
2
S can be applied
as an effective probe for the visualization and study of H2S in mitochondria and in vivo.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.