Forest fire, as a discontinuous ecological factor of forest, causes the changes of carbon storage and carbon distribution in forest ecosystem, and affects the process of forest succession and national carbon capacity. Taking the burned land with different forest fire interference intensity as the research object, using the comparison method of adjacent sample plots, and taking the combination of field investigation sampling and indoor test analysis as the main means, this paper studies the influence of different forest fire interference intensity on the carbon pool of forest ecosystem and the change and spatial distribution pattern of ecosystem carbon density, and discusses the influence mechanism of forest fire interference on ecosystem carbon density and distribution pattern. The results showed that forest fire disturbance reduced the carbon density of vegetation (P < 0.05). The carbon density of vegetation in the light, moderate and high forest fire disturbance sample plots were 67.88, 35.68 and 15.50 t∙hm-2, which decreased by 15.86%, 55.78% and 80.79% respectively compared with the control group. In the light, moderate and high forest fire disturbance sample plots, the carbon density of litter was 1.43, 0.94 and 0.81 t∙hm-2, which decreased by 28.14%, 52.76% and 59.30% respectively compared with the control group. The soil organic carbon density of the sample plots with different forest fire disturbance intensity is lower than that of the control group, and the reduction degree gradually decreases with the increase of soil profile depth. The soil organic carbon density of the sample plots with light, moderate and high forest fire disturbance is 103.30, 84.33 and 70.04 t∙hm-2 respectively, which is 11.670%, 27.899% and 40.11% lower than that of the control group respectively; the carbon density of forest ecosystem was 172.61, 120.95 and 86.35 t∙hm-2 after light, moderate and high forest fire disturbance, which decreased by 13.53%, 39.41% and 56.74% respectively compared with the control group; forest fire disturbance reduced the carbon density of eucalyptus forest, which showed a law of carbon density decreasing with the increase of forest fire disturbance intensity. Compared with the control group, the effect of light forest fire disturbance intensity on the carbon density of eucalyptus forest was not significant (P > 0.05), while the effect of moderate and high forest fire disturbance intensity on the carbon density of eucalyptus forest was significant (P < 0.05).
Heat stress due to external heat sources such as fire is an ecological problem for plants. When forest plants suffer from fire, high temperatures cause an array of morphological, physiological, and biochemical changes, which affect growth and development. Michelia macclurei Dandy is an evergreen broad-leaved tree species with the characteristics of fast growth, strong adaptability, and good fire-resistance. Some studies have improved the understanding of how fire behavior affects physiology, function and mortality, but the extreme heat response genes and mechanisms need improved understanding. In this study, we conducted a fire experiment (slight and severe) and RNA-Seq in M. macclure. The de novo assembly obtained 104,052 unigenes, and 48.46% were annotated in at least one public database. Specifically, 4458 and 4810 differentially expressed genes (DEGs) were identified in slight and severe fire treatment groups, respectively. In two treatment groups, 612 unigenes were differentially expressed, which were enriched in ‘oxidoreductase activity’ in the molecular function (MF) category of Gene Ontology (GO) enrichment analysis, suggesting the core role of oxidoreductase activity in response to extremely high temperatures in M. macclurei. In KEGG enrichment analysis of DEGs, the ‘plant hormone signal transduction’ is overrepresented, suggesting that this process plays an important role during heat response in M. macclurei. In the pathways of cytokinine and salicylic acid, some vital DEGs were enriched, which were related to cell division, shoot initiation, and disease resistance, and the potential interactions during heat stress were discussed. Moreover, the DEGs linked to heat stress response were identified, including heat shock factors, stress enhanced protein, signal transduction, photosystem, and major transcription factors. The qRT-PCR examination of various tissues, expression dynamics, and treatments revealed that the genes coding for the heat shock protein HSF30, stress enhanced protein, and photosystem I reaction center subunit II exhibited particularities in leaf tissue. Genes coding for heat shock proteins displayed a distinct expression pattern between fire treatment and conventional heat stress, which could signify the distinctive function of HSPs and the mechanism of heat responses. Altogether, these may interact to respond to fire stress through alterations in cellular processes, signaling transduction, and the synthesis and degradation of response proteins in M. macclurei. The results of this study provide a crucial transcriptional profile influenced by heat stress in M. macclurei, and could be of great use to explore the fire prevention mechanisms of fire-resistant tree species.
Michelia macclurei (Dandy, 1928) is an evergreen broad-leaved tree species native to South China. This species has great ecological and economic importance. However, the genomic study of M. macclurei has lagged far behind. Here, we reported the complete chloroplast genome sequence of M. macclurei . The chloroplast genome size of M. macclurei was 160,139 bp, consisting of a pair of inverted repeat (IR) regions (26,575 bp), which was separated by a large single copy (LSC) region (88,167 bp) and a small single copy (SSC) region (18,822 bp). A total of 113 unique genes were annotated, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. The overall GC content was 39.2%. Phylogenetic analysis based on 16 whole chloroplast genome sequences of Michelia species suggested that M. macclurei and M. maudiae are sister to each other, and jointly sister to M. chapensis .
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