Purpose: Long intergenic non-protein coding RNA 1224 (LINC01224) plays vital roles in the tumorigenesis and progression of hepatocellular carcinoma. Here, we determined LINC01224 expression in epithelial ovarian cancer (EOC) tissues and cells. We also assessed the effects of LINC01224 knockdown on the malignant phenotype of EOC cells both in vitro and in vivo. Furthermore, the detailed molecular mechanisms underlying the oncogenic actions of LINC01224 in EOC cells were elucidated. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect LINC01224 expression in EOC tissues and cells. EOC cells were transfected with small interfering RNAs, and cell proliferation, apoptosis, migration, and invasion were assessed using Cell Counting Kit-8 assay, flow cytometry, cell migration assays, and cell invasion assays, respectively. Using tumor xenografts, the effects of LINC01224 silencing on EOC tumor growth were analyzed in vivo. The mechanism underlying LINC01224 regulation of malignant processes in EOC cells was explored using bioinformatics, RNA immunoprecipitation assay, qRT-PCR, Western blotting, and rescue experiments. Results: LINC01224 expression was upregulated in EOC tissues and cells. LINC01224 upregulation was correlated to tumor size, the International Federation of Gynecology and Obstetrics stage, and lymph node metastasis. LINC01224 depletion in EOC cells suppressed cell proliferation, migration, and invasion and facilitated cell apoptosis in vitro. LINC01224 downregulation also hindered EOC tumor growth in vivo. Mechanistically, LINC01224 served as a competing endogenous RNA for microRNA-485-5p (miR-485-5p) and consequently increased p21-activated kinase 4 (PAK4) expression in EOC cells. Furthermore, miR-485-5p inhibition or PAK4 upregulation significantly abrogated the effects of LINC01224 depletion in EOC cells. Conclusion: LINC01224/miR-485-5p/PAK4 formed a competing endogenous RNA network regulating the aggressive behavior of EOC. Therefore, targeting this pathway may be an attractive therapeutic strategy for EOC.
We propose an interferometric method that enables to measure a distance by the intensity measurement using the scanning of the interferometer reference arm and the recording of the interference fringes including the brightest fringe. With the consideration of the dispersion and absorption of the pulse laser in a dispersive and absorptive medium, we investigate the cross-correlation function between two femtosecond laser pulses in the time domain. We also introduce the measurement principle. We study the relationship between the position of the brightest fringe and the distance measured, which can contribute to the distance measurement. In the experiments, we measure distances using the method of the intensity detection while the reference arm of Michelson interferometer is scanned and the fringes including the brightest fringe is recorded. Firstly we measure a distance in a range of 10 µm. The experimental results show that the maximum deviation is 45 nm with the method of light intensity detection. Secondly, an interference system using three Michelson interferometers is developed, which combines the methods of light intensity detection and time-of-flight. This system can extend the non-ambiguity range of the method of light intensity detection. We can determine a distance uniquely with a larger non-ambiguity range. It is shown that this method and system can realize absolute distance measurement, and the measurement range is a few micrometers in the vicinity of Nl(pp), where N is an integer, and lpp is the pulse-to-pulse length.
High-accuracy distance measurement plays an important role in many applications, such as industry measurement, Aerospace and scientific research. The continual development oflaser ranging technique is always a frontier topic of geometric measurement, therefore this paper develops the application of femtosecond optical frequency comb for length measurement. Time-of-flight principle for distance measurement is widely used by pulse laser, however, achievable resolution reaches only a few millimeters at best due to the limited bandwidth of electronics. In this paper, the temporal coherence of multiple pulse train is analyzed, and an arbitrary and absolute length measurement system is set up based on modified Michelson interferometer by combining multiple pulse train interference and time-of-flight method. The timing difference is separately calculated by the first-order and second-order optical cross-correlation signals, therefore the absolute distance is calculated. An experiment is conducted by measuring a 60 cm length, and the measurement result is compared with a high precision laser displacement sensor. The result shows a good measuring linearity, and the measurement precision of ±0.5 μm is achieved.
The postpartum hemorrhage increases with the increasing use of cesarean section, one of the common methods of delivery. In order to improve the postoperative safety of women with cesarean section, an effective strategy to prevent bleeding after cesarean section is extremely important. This study compares the preventive effects of carboprost with carboprost tromethamine and carboprost with oxytocin on postpartum hemorrhage of cesarean section, which will provide reference for clinical effective prevention of bleeding after cesarean section. A prospective analysis was performed on 349 cases of cesarean section puerperants. According to the treatment method, they were divided into two groups: carboprost suppository combined with oxytocin group (control group) and carboprost suppository combined with romethamine group (study group). The general data, hemodynamic parameters [heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP) and blood oxygen saturation(Sp02)], incidence of adverse reactions, bleeding, uterine recovery and drug efficacy of the patients were compared. There were no differences in hemodynamic indexes between the two groups before medication (P> 0.050). After medication, the HR, SBP, and DBP of the study group were lower than those of the control group, and Sp02 of the study group was higher than that of the control group (P <0.001). The HR and Sp02 of the two groups after medication were higher than those before medication, while the SBP and DBP were lower than those before medication (P <0.050). The total postoperative bleeding volume in the study group was lower than that in the control group (P <0.05); the postoperative bleeding rate in the study group was lower than that in the control group (P <0.05). The uterine return in the study group was better than that in the control group (P <0.05). The total effective rate of the control group was higher than that of the control group (P<0.05). The combination of carboprost suppository and romethamine can better prevent the bleeding after cesarean section, and it is worth popularizing and developing.
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