Currently, the prognosis of acute myeloid leukemia (AML) is poor. In the AML microenvironment, bone marrow (BM) mesenchymal stem cells (BMMSCs) serve an important role in protecting AML cells from chemotherapy-induced apoptosis. The present study aimed to evaluate the expression of fibroblast activation protein α (FAPα) in BMMSCs and BM biopsy samples via flow cytometry, reverse transcription-quantitative PCR and immunohistochemistry, as well as to identify the correlation between the expression of FAPα in BM with clinical parameters and survival of newly diagnosed patients with AML. Subsequently, the protective effect of FAPα on Cytosine arabinoside (Ara-C)-induced apoptosis in Kasumi-1 cells was investigated via small interfering (si)RNA, and its underlying mechanism was examined by western blotting. The results demonstrated significant differences in FAPα expression in BMMSCs and BM biopsy samples between patients with AML and healthy donors. Furthermore, BMMSCs protected Ara-C-induced Kasumi-1 cells from apoptosis, and knockdown of FAPα using siRNA decreased this protection. It was found that Kasumi-1 cells expressed β-catenin, which could be inhibited by Ara-C, and β-catenin expression was significantly activated when co-cultured with BMMSCs, even in the presence of Ara-C. Knockdown of FAPα with siRNA significantly suppressed the expression of β-catenin. The present results indicated that FAPα serves an important role in the AML BM microenvironment, and that increased expression of FAPα in BM may be a poor prognostic factor in patients with AML. Moreover, the current findings demonstrated that BMMSCs protected AML cells from apoptosis, which was in part contributed by FAPα, and may occur via the β-catenin signaling pathway.
Background:In view of the complexity of tumorigenesis, it is very important to conduct a pan-cancer expression analysis and assess its correlation with clinical prognosis and potential molecular mechanisms of gene interested. More and more evidences show that HK2 is closely related to tumors. But no pan-cancer analysis is available. The aim of this paper was to explore the potential roles of HK2 across thirty-three tumors based on the datasets of TCGA (The Cancer Genome Atlas) and GEO (Gene Expression Omnibus). Results:HK2 is highly expressed in most tumors and related to the progression of some tumors. HK2 expression were associated with the infiltration of T follicular helper cells (Tfh) for the TCGA tumors of UVM, BRCA, BRCA-LumA, HNSC, HNSC-HPV+, and cancer-associated fibroblasts for the tumors of LGG and STAD. Conclusion:Our first pan-cancer analyses of HK2 indicated statistical correlations of HK2 expression with clinical prognosis and immune cell infiltration across multiple tumors, which help to understand the role of HK2 in tumorigenesis from the perspective of clinical tumor samples.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.