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Introduction With growing awareness of the link between diet and health and the problem of obesity, public concern over sugar levels in the diet is forcing a worldwide trend toward cutting down on sugar by using artificial sweeteners (AS). Aim To study the effect of increasing concentrations of sucralose (an AS) on glucose uptake in rat L6 myotubes. Materials and methods The L6 cell line from American type cell culture (ATCC) was grown in Dulbecco's Modified Eagle's Medium (DMEM) and differentiated into myotubes. The wells were exposed to either 0, 1 nM, 1 μM, or 1 mM of sucralose alone or with 10 nM insulin for 24 hours. Glucose uptake was studied after this period. Results Significant decrease was seen between the insulin-stimulated basal glucose uptake and insulin-stimulated glucose uptake across all the concentrations of sucralose treatment. Conclusion Increased concentration of sucralose appears to decrease glucose uptake even on insulin stimulation. Clinical significance It may not be beneficial to use sucralose in certain groups of people who have insulin resistance or are prone to it. How to cite this article Prakash SN, Shanthakumari J, Devanath A. Effect of Sucralose on Glucose Uptake in Rat L6 Myotubes. Indian J Med Biochem 2017;21(2):162-165.

Aim and objectives: To compare and evaluate the effect of sample type on vitamin D concentration in order to avoid multiple sample collection. Materials and methods: The study was conducted in a tertiary care hospital on 40 adult subjects whose samples were for both serum vitamin D and plasma parathyroid hormone (PTH). Leftover plasma sample was utilized for vitamin D analysis. Samples were analyzed on Advia Centaur XP from Siemens Healthineers. Bland-Altman analysis and regression equation were derived to evaluate the extent of agreement and conversion between sample types. Results and conclusion: Serum vitamin D is higher than plasma vitamin D (by 6.5 ng/mL), and it is more pronounced in samples with vitamin D greater than 20 ng/mL. Clinical significance: It is important that lab and clinicians should be aware of the comparably large preanalytical bias introduced by changing between serum and EDTA plasma sample for vitamin D analysis. This would impact patients who are serially monitored for vitamin D supplementation.

Polymorphism in Vitamin D binding protein (DBP) has been implicated as one of the causes for Vitamin D deficiency. However there is paucity of data regarding the effect of genetic polymorphism in DBP in Vitamin D deficient patients in our population. This pilot study was undertaken to analyze the common genetic polymorphism in vitamin DBP in these population and its effect on vitamin D supplementation. 80 vitamin D deficient subjects were selected by convenient sampling. Genetic analysis for DBP (GC) gene polymorphism (rs7041 + rs4588) was carried out in all these individuals after informed consent and correlated with the vitamin D levels post supplementation. Six combinations of genotype were obtained (rs7041 + rs4588): TT+CA, TG+CC, TT+CA, TT+CC, TT+AA, GG+CC. A third of all individuals (33%) were found to have the TG+CA genotype, followed by about 26 % of individuals having the GG+CC genotype. TT+CA group was found to have 13% individuals and a tenth of all individuals belonged to each of the groups with TG+CC and TT+AA genotypes. Least proportion of individuals was found to have the TT+CA genotype (6%). There was no significant difference in the vitamin D levels with individual polymorphism (p value <0.01). However the combined genotype had an effect, with homozygotes for both such as TT-CC, TT-AA showing least response and heterozygotes such TG-CA and CG-CC showing better response: In this study, the individual SNP (rs7041 and rs4588) did not seem to significantly influence the response to vitamin D supplementation. However the combined genotype seem ed to influence the proportion of patients showing improvement after supplementation. The homozygotes for both such as TT-CC, TT-AA showing least response and heterozygotes such TG-CA and CG-CC showing better response.