The mitochondrial genome may include crucial data for understanding phylogenetic and molecular evolution. We sequenced the complete mitogenome of Haemaphysalis nepalensis and Haemaphysalis yeni for the first time. H. nepalensis and H. yeni's complete mitogenomes were 14,720 and 14,895 bp in size, respectively, and both contained two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 13 protein-coding genes (PCG). Haemaphysalis nepalensis have one control region (D-loop). The adenine + thymine concentration of the genomes of H. nepalensis and H. yeni was 77.75 and 78.41%, respectively. The codon use pattern and amino acid content of proteins were both observed to be affected by the AT bias. Genes in the mitogenome were organized and located in a comparable manner to previously known genes from Haemaphysalis ticks. Mitochondrial PCGs were used to perform phylogenetic relationships based on the Minimum Evolution (ME) approach using MEGA 7.0 software, the results reveal that H. nepalensis has tight links with H. tibetensis, H. yeni and H. kolonini share a sister group relationship, and that H. nepalensis and H. yeni belong to Haemaphysalis. The results of this study include the following: (i) discovered and supplied new tick records (H. nepalensis) for China, (ii) provided the first complete mitochondrial genome for H. nepalensis and H. yeni and revealed their phylogenetic relationships, and (iii) the features of the mitochondrial genome of H. nepalensis and H. yeni provided more genetic reference for Phylogeography, systematics, and population genetics of the Haemaphysalis species.
In order to explore the reasonable ore blending of low-silicon magnetite in sintering, it I necessary to realize the efficient utilization of low-silicon ore, further reduce cost, and increase yield. In this study, based on the high-temperature basic characteristics of iron ore powder used in the experiment, sinter pot tests were carried out with different low-silicon ore ratios, and the microstructure of the sinter was observed by scanning electron microscopy (SEM) and energy spectrum analysis (EDS) to determine the optimal matching law of low-silicon ore. The result showed that SiO2, Al2O3, and burning loss in iron ore powder composition were positively correlated with its assimilation, whereas MgO and basicity R2 were negatively correlated with the assimilation of iron ore powder. When the ratio of low-silicon ore was not more than 35%, increasing the ratio of hematite improved the liquid production and increased the production of acicular calcium ferrite. Therefore, the optimization of ore blending based on assimilation can improve the quality of sinter and strengthen the sintering process. This study has certain reference significance for the industrial production of low-silica sintering.
In this paper, hot-dip aluminizing of ferrite nodular cast iron was carried out after treating liquid aluminum with different electrical pulse parameters. Compared with that of conventional hot-dip aluminizing, the coating structure of the treated sample did not change, the surface was smooth and continuous, and the solidification structure was more uniform. When high voltage and large capacitance were used to treat the liquid aluminum, the thickness and compactness of the coating surface layer increased. The thickness of the alloy layer decreased, and, the compactness and the micro hardness increased, so the electric pulse had a certain inhibition on the formation of the alloy layer. The growth kinetics of the alloy layer showed that the rate-time index decreased from 0.60 for the conventional sample to 0.38 for the electric pulse treated sample. The growth of the alloy layer was controlled by diffusion and interface reaction, but only by diffusion. The AC impedance and polarization curves of the coating showed that the corrosion resistance of hot-dip coating on nodular cast iron was improved by electric pulse treatment.
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