This study detected the upstream and downstream key genes of glycolysis in Dunaliella Salina by using Real-time FluorescenceQuantitative PCR assays and measurement of enzyme activity. The results were as follows: the levels of transcription, enzyme activity, and protein of D. salina PFK were up-regulated under hyperosmotic stress while D. salina ENO were down-regulated. At the same time we monitored the change of intracellular degradation of starch, the synthesis of glycerol and PEP concentration in Dunaliella Salina under hyperosmotic stress. We found that lower expression of DsENO reduced the concentration of intracellular PEP which promoted the degradation of starch, and decreased the flow of carbon into the tricarboxylic acid cycle which would favor the synthesis of glycerol.
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