The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.
Background Intramuscular fat (IMF) content is the major evaluating indicator in chicken meat quality. We previously found that miR-128-3p was differentially expressed in chicken intramuscular adipocyte at different periods. However, little is known about miR-128-3p associated with intramuscular adipocyte differentiation. The purpose of this present study was to investigate the biological functions and regulatory mechanism of miR-128-3p in chicken intramuscular adipogenesis. Results A series of assay results confirmed that miR-128-3p could promote the proliferation of intramuscular adipocytes and inhibit cell differentiation. A total of 223 and 1,050 differentially expressed genes (DEGs) were identified in the mimic treatment group and inhibitor treatment group compared with the control group, respectively. Functional enrichment analysis revealed that DEGs involved in lipid metabolism related processes, such as MAPK signaling pathway and TGF-β signaling pathway. Furthermore, target genes prediction analysis showed that miR-128-3p can target multiple genes from DEGs, such as FDPS, GGT5, TMEM37, and ASL2. Luciferase assay showed that the miR-128-3p targeted to the 3’ UTR of FDPS. Subsequent functional assays demonstrated that miR-128-3p acted as an inhibitor of intramuscular adipocyte differentiation by targeting FDPS. Conclusion This study found that miR-128-3p inhibited chicken intramuscular adipocytes differentiation by down regulating FDPS. Our findings provided a theoretical basis for the study of lipid metabolism, and also provided a potential target for molecular breeding to improve meat quality.
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