Knowledge of soil moisture spatial variation with land use along the precipitation gradient is necessary to improve land management and guide restoration practice in the water‐limited Chinese Loess Plateau. This study selected 45 sampling points at 11 sites across the north–south transect of the Loess Plateau based on the precipitation gradient and land use. Results showed that the vertical profiles of soil moisture revealed large variations with the precipitation gradient changing, especially in the surface layer (0–100 cm). Significant linear correlation existed between the average soil moisture of the profile and the mean annual precipitation (MAP) for each land use type (p < 0·05). Hereinto, the soil moisture under the grassland was affected more greatly by precipitation. The soil moisture under each land use commonly revealed the trend as farmland > grassland > shrubland > woodland, while it might be higher under the woodland than the shrubland in the surface layer in regions with MAP <500 mm. The soil moisture of woodland or shrubland at the selected points was below or approximate to the permanent wilting point in regions with MAP <520 mm. Covariance analysis confirmed the effects of land use and MAP on the soil moisture in depth of 100–300 cm, and it showed land use did not pose significant effects in the surface layer. In addition, our study indicated that it is necessary to reconsider and re‐evaluate the current vegetation restoration strategy in the perspective of vegetation sustainability and soil water availability, in which woodland and shrubland were selected on a large scale in the arid and semi‐arid regions. Copyright © 2016 John Wiley & Sons, Ltd.

Histone deacetylase enzymes participate in the regulation of many aspects of plant development. However, the genome-level targets of histone deacetylation during apple (Malus domestica) fruit development have not been resolved in detail, and the mechanisms of regulation of such a process are unknown. We previously showed that the complex of ethylene response factor 4 (MdERF4) and the TOPLESS co-repressor (MdTPL4) (MdERF4-MdTPL4) is constitutively active during apple fruit development, but whether this transcriptional repression complex is coupled to chromatin modification is unknown. Here, we show that a histone deacetylase (MdHDA19) is recruited to the MdERF4-MdTPL4 complex, thereby impacting fruit ethylene biosynthesis. Transient suppression of MdHDA19 expression promoted fruit ripening and ethylene production. To identify potential downstream target genes regulated by MdHDA19, we conducted chromatin immunoprecipitation (ChIP) sequencing of H3K9 and ChIP-quantitative PCR assays. We found that MdHDA19 affects ethylene production by facilitating H3K9 deacetylation and forms a complex with MdERF4-MdTPL4 to directly repress MdACS3a expression by decreasing the degree of acetylation. We demonstrate that an early-maturing-specific acetylation H3K9ac peak in MdACS3a and expression of MdACS3a were specifically up-regulated in fruit of an early-maturing, but not a late-maturing, cultivar. We provide evidence that a C-to-G mutation in the EAR motif of MdERF4 reduces the repression of MdACS3a by the MdERF4-MdTPL4-MdHDA19 complex. Taken together, our results reveal that the MdERF4-MdTPL-MdHDA19 repressor complex participates in the epigenetic regulation of apple fruit ripening.

As apple fruits (Malus domestica) mature, they accumulate anthocyanins concomitantly with losing chlorophyll (Chl); however, the molecular pathways and events that coordinate Chl degradation and fruit coloration have not been elucidated. We showed previously that the transcription factor ETHYLENE RESPONSE FACTOR17 (MdERF17) modulates Chl degradation in apple fruit peels and that variation in the pattern of MdERF17 serine (Ser) residues is responsible for differences in its transcriptional regulatory activity. Here, we report that MdERF17 interacts with and is phosphorylated by MAP KINASE4 (MdMPK4-14G). Phosphorylation of MdERF17 at residue Thr67 by MdMPK4-14G is necessary for its transcriptional regulatory activity and its regulation of Chl degradation. We also show that MdERF17 mutants with different numbers of Ser repeat insertions exhibit altered phosphorylation profiles, with more repeats increasing its interaction with MdMPK4. MdMPK4-14G can be activated by exposure to darkness and is involved in the dark-induced degreening of fruit peels. We also demonstrate that greater phosphorylation of MdERF17 by MdMPK4-14G is responsible for the regulation of Chl degradation during light/dark transitions. Overall, our findings reveal the mechanism by which MdMPK4 controls fruit peel coloration.

No abstract

Soil moisture pulses are a prerequisite for other land surface pulses at various spatiotemporal scales in arid and semi-arid areas. The temporal dynamics and profile variability of soil moisture in relation to land cover combinations were studied along five slopes transect on the Loess Plateau during the rainy season of 2011. Within the 3 months of the growing season coupled with the rainy season, all of the soil moisture was replenished in the area, proving that a type stability exists between different land cover soil moisture levels. Land cover combinations disturbed the trend determined by topography and increased soil moisture variability in space and time. The stability of soil moisture resulting from the dynamic processes could produce stable patterns on the slopes. The relationships between the mean soil moisture and vertical standard deviation (SD) and coefficient of variation (CV) were more complex, largely due to the fact that different land cover types had distinctive vertical patterns of soil moisture. The spatial SD of each layer had a positive correlation and the spatial CV exhibited a negative correlation with the increase in mean soil moisture. The soil moisture stability implies that sampling comparisons in this area can be conducted at different times to accurately compare different land use types.

H9N2 subtype avian influenza viruses are widespread in domestic poultry. Genetic analysis indicated that three lineages of H9N2 viruses have been established in Eurasia and only one lineage is present on chicken farms in mainland China. Here, NS1 genes of eight H9N2 chicken influenza viruses, isolated in mainland China during 1998-2002, were completely sequenced and phylogenetically analyzed. By comparison, the homology of the NS1 of the A/chicken/Neimenggu/ZH/02 (Ck/NM/ZH/02) strain had a high identity (93.8%) with that of A/chicken/Korea/323/96 (Ck/Kor/323/96), which is an A/duck/Hong Kong/Y439/97 (Dk/HK/Y439/97)-like virus. NS1 peptides of seven strains possessed 217 amino acids, while that of the strain Ck/NM/ZH/02 coded 230 amino acids. Except for the amino acid at position 225, the additional amino acid sequence (13 AAs) of NS1 of Ck/NM/Zh/02 at the carboxy-terminus is identical with that of Ck/Kor/323/96. Phylogenetic analysis showed that seven of the tested strains belong to the A/duck/Hong Kong/Y280/97 (DK/HK/Y280/97)-like lineage, while the NS1 gene of Ck/NM/Zh/02 belongs to the Dk/HK/Y439/97-like lineage and has a close relationship with that of the Ck/Kor/323/96-like viruses. Therefore, although most of the H9N2 influenza viruses circulating on chicken farms in mainland China belong to the DK/HK/Y280/97-like lineage, the present results indicate that the other two of the three H9N2 lineage viruses also circulate in the chicken population in mainland China.

Abstract. The temporal and spatial distributions of regional irrigation water productivity (RIWP) are crucial for making decisions related to agriculture, especially in arid irrigated areas with complex cropping patterns. Thus, in this study, we developed a new RIWP model for an irrigated agricultural area with complex cropping patterns. The model couples the irrigation- and drainage-driven soil water and salinity dynamics and shallow groundwater movement in order to quantify the temporal and spatial distributions of the target hydrological and biophysical variables. We divided the study area into 1 km × 1 km hydrological response units (HRUs). In each HRU, we considered four land use types: sunflower fields, wheat fields, maize fields, and uncultivated lands (bare soil). We coupled the regional soil hydrological processes and groundwater flow by taking a weighted average of the water exchange between unsaturated soil and groundwater under different land use types. The RIWP model was calibrated and validated using 8 years of hydrological variables obtained from regional observation sites in a typical arid irrigation area in North China, the Hetao Irrigation District. The model simulated soil moisture and salinity reasonably well as well as groundwater table depths and salinity. However, overestimations of groundwater discharge were detected in both the calibration and validation due to the assumption of well-operated drainage ditch conditions; regional evapotranspiration (ET) was reasonably estimated, whereas ET in the uncultivated area was slightly underestimated in the RIWP model. A sensitivity analysis indicated that the soil evaporation coefficient and the specific yield were the key parameters for the RIWP simulation. The results showed that the RIWP decreased from maize to sunflower to wheat from 2006 to 2013. It was also found that the maximum RIWP was reached when the groundwater table depth was between 2 and 4 m, regardless of the irrigation water depth applied. This implies the importance of groundwater table control on the RIWP. Overall, our distributed RIWP model can effectively simulate the temporal and spatial distribution of the RIWP and provide critical water allocation suggestions for decision-makers.

No abstract