Transformation of metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-l-methylethyl)-acetamide] was studied with an actinomycete strain isolated from metolachlor-contaminated soil. Eight metabolites were obtained and identified by mass and NMR spectral analysis. Benzylic hydroxylation of the aralkyl side chains and/or demethylation a t the N-alkyl substituent appeared to be the only transformations involved. All metabolites had a monochlorine isotopic pattern, indicating that no dehalogenation of the chloroacetyl moiety occurred.
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