Nattokinase (NK) is a potent fibrinolytic enzyme , which belongs to the second large family of serine proteases. NK gain significant attention in the treatment of hypertension and cardiovascular disorders (CVD's). Thus, a number of NK producers have been extensively studied, especially Pseudomonas sp. has extended the production level than Bacillus sp. with different properties. Thus the current study precedes with the strain improvement of NK producing UV mutated Pseudomonas aeruginosa CMSS by chemical mutagenesis. The potent mutant strain UV-EMS, treated with Ethyl Methyl Sulphonate (EMS) showed maximum NK activity. The maximum production of NK from mutant strain was determined at optimized parameters like pH 5 (1315.8 U mL-1), temperature at 37 o C (2413.3 U mL-1), shrimp shell as nitrogen source (2355.0 U mL-1) and sucrose as carbon source (3930.0 U mL-1).The activity of partially purified NK was stable at pH 7, temperature at 10 o C and 5mM of MgCl 2. The stability of NK was partially inhibited by SDS and completely inhibited by EDTA. The partially purified NK showed74% of in vitro clot lysis activity.
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