Background: Escherichia coli and Klebsiella spp. are the most common organisms causing urinary tract infection (UTI) and commonly responsible for extended spectrum beta lactamase (ESBL) production. This study was carried out to detect ESBL producing uropathogenic Escherichia coli and Klebsiella spp. by phenotypic and genotypic method. Rapid and accurate detection of ESBL producing E. coli and Klebsiella spp. has an important role to avoid treatment failure. Materials and Methods: This was a cross sectional observational study and carried out in department of microbiology in Chittagong Medical College, Bangladesh from January to December 2017. Urine was collected from suspected UTI patients and standard microbiological and biochemical tests were carried out. ESBL producing E. coli and Klebsiella spp. were identified by phenotypic confirmatory disc diffusion test (PCDDT). Polymerase chain reaction (PCR) was performed by using standard protocol with specific primers. Results: 448 urine samples were collected. Among them, 140 showed bacterial growth; 72 were E. coli and 35 were Klebsiella spp. Among E. coli 38(52.8%) and in Klebsiella spp. 15(42.9%) were detected as ESBL producers by PCDDT respectively. Among E. coli, 41(56.9%) and in Klebsiella spp. 21(60%) strains produced ESBL genes by PCR. Out of 38 phenotypically positive E. coli, 7 strains do not carry any detectable genes. Similarly, out of 15 phenotypically positive Klebliella spp. 3 isolates did not produce any detectable gene. On the other hand, 10 E. coli isolates and 9 Klebsiella spp. carry detectable genes although these were not phenotypically ESBL producers. Moreover, ESBL producing E. coli and Klebsiella spp. showed more multidrug resistant than Non-ESBL producing E. coli and Klebsiella spp. Conclusion: This study revealed that large portion of E. coli and Klebsiella spp. was ESBL producers. PCR can detect some additional cases of ESBL producing isolates. So, PCR can be used along with phenotypic ......
Background: Urinary tract infection is one the most common infection in clinical practice. Uropathogens have the ability to form biofilm in urinary tract, frequently within the indwelling catheter. Microorganism growing in a biofilm is associated with chronic and recurrent UTI and less sensitive to antimicrobial agent. So, the aim of the present study was to detect biofilm-producing uropathogenic bacteria by microtiter plate assay and antibiotic sensitivity pattern of biofilm- producing and biofilm non-producing organisms. Methods: This cross- sectional observational study was carried out in Microbiology Department, Chattogram Medical College, Bangladesh. Urine samples were collected from outpatient’s department and inpatients of different wards. Standard microbiological procedures and biochemical tests were carried out. Antibiotic susceptibility test was performed by using the Kirby-Bauer disk diffusion technique. Biofilm production was detected by Microtiter plate method (MPM). Results: Out of 252 tested samples, 73(55.3%) organisms were isolated from non-catheterized urine and 74(61.66%) from catheterized urine samples. The most frequently isolated organism was Escherichia coli (60.27%, 50%) in both non- catheterized and catheterized patients followed by Klebsiella spp. (21.91%, 27.02%); Pseudomonas spp. (9.58%, 12.21%); Acinetobacter spp. (1.36%, 4.05%); Staphylococcus aureus (4.1%, 2.7%) respectively and 2.7% CoNS from non-catheterized patients. In the non- catheterized patients, 19 (26.02%) out of 73 bacterial isolates were biofilm-forming and in the catheterized patients, 33 (44.59%) out of 74 bacterial isolates were biofilm forming. The maximum biofilm-producing organism was Escherichia coli in both isolates. Biofilm- producing organism found relatively high resistance against tested antibiotics. Imipenem, Amikacin, Nitrofurantoin, and Piperacillin-tazobactam are the few microbial agents that are effective against biofilm-producing gram-negative organisms ..
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.