ObjectiveTo investigate the effects of NK4 gene on the properties and tumorigenicity in laryngeal squamous cell carcinoma cell.MethodsHere, we used the attenuated Salmonella carrying the NK4 gene to transfect the AMC-HN-8 cells and detected the expression of NK4 by the real-time quantitative polymerase chain reaction (q RT-PCR). The properties of NK4 gene was determined by MTT method, cell scratch test, and flow cytometry. A nude mouse tumorigenesis model was used to evaluate the effect of NK4 gene on the growth of AMC-HN-8 cells in vivo. While a western blot assay was used to assess the expression of DKK1, Wnt1 and β-Catenin in nude mouse tumors.ResultsqRT-PCR showed that the expression of NK4 in the transfection group was significantly higher than that in the control group (P<0.01), and the expression increased with the time of transfection. MTT results showed NK4 overexpression inhibited the proliferation of AMC-HN-8 cells, and the inhibitory activity no longer increased with increasing dose when 30% expression supernatant was added (P<0.01). Scratch experiment showed that NK4 overexpression decreased the cell migration ability (P<0.01). Annexin V/PI double staining experiment showed that NK4 gene induced AMC-HN-8 cell apoptosis (P<0.01), and cell cycle arrest in S phase (P<0.01). NK4 overexpression inhibited tumor formation ability of AMC-HN-8 cells in vivo (P <0.05). WB detection showed that the expression of DKK1 increased, Wnt1 and β-Catenin protein decreased after the high expression of NK4.ConclusionsNK4 gene inhibit cell proliferation and migration, while promote cell apoptosis, and induce cell cycle arrest in S phase of laryngeal carcinoma AMC-HN-8 cells. NK4 overexpression inhibit the tumorigenesis ability of AMC-HN-8 cells, which may be related to the regulation of DKK1/Wnt1/β-Catenin signal axis.
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Background: To better explore the underlying mechanism of liver metastatic formation by placenta-specific protein 1 (PLAC1) in human colorectal cancer, we investigated the proliferation, invasion and angiogenic capabilities of human colorectal cancer cell lines with different liver metastatic potentials as well as the mechanism of action of PLAC1 in the metastatic process. Methods: The expression of PLAC1 was detected by reverse transcriptase PCR, western blot and real-time PCR. The effect of PLAC1 on metastatic potential was determined by proliferation, invasion, and angiogenesis assays, including an in vitro coculture system consisting of cancer cells and vascular endothelial cells that were used to detect the relationship between cancer cells and angiogenesis. In addition, we also determined PLAC1 downstream targets that preferentially contribute to the metastatic process. Results: PLAC1 was expressed in HT-29, WiDr and CaCo-2 colorectal cancer cells but not in Colo320 colorectal cancer cells. PLAC1 could not only significantly enhance the proliferation of CoLo320 and human umbilical vein endothelial cells (HUVECs) but could also promote the invasion of CoLo320 cells. The angiogenesis of HUVECs was enhanced by PLAC1 in a dose-dependent manner. In cocultured systems, angiogenesis was significantly increased by coculture with HT-29 cells. In addition, PLAC1 could promote angiogenesis in coculture with HT-29 cells. Furthermore, PLAC1-enhanced metastatic potential of colorectal cancer cells was dependent on activation of the PI3K/Akt/NF-κB pathway. Conclusions: The activation of PI3K/Akt/NF-κB signaling by PLAC1 may be critical for the metastasis of colorectal cancer cells. According to our results, we suggest that modification of PLAC1 function might be a promising new therapeutic approach to inhibit the aggressive spread of colorectal cancer.
The widely recognized industry standard IPC-7525 has been used as the starting point for an experimental program that explores the effect of varying the keep out distance for 0201 and 0402 chip components, CSP and SOP with pitches down to OAmm, and larger components represented by CCGA. Other variables that were included in the experimental program to determine if they had an effect on the sensitivity of paste transfer to keep-out distance included stencil type, step height and solder type.In the first stage of the project the printing to each pad was measured with automated 3D SPI systems and optimum combinations of parameters identified by statistical analysis. In this paper the authors will explain the methodology chosen to achieve the project objectives and indicate the direction of likely future work.Early results indicate that a key objective of the project, to provide evidence to support the case for a reduction in the keep out distances below the current industry standard, might be achievable.
OBJECTIVE To systematically evaluate the efficacy and safety of montelukast sodium combined with budesonide nasal spray in the treatment of allergic rhinitis.METHODS Databases such as China Biomedical Literature Database, China Knowledge Network, Wanfang Database, VIP Database, Embase, Cochrane Library, Pubmed, and Web Of Science were searched. Randomized controlled trials evaluating montelukast sodium in combination with budesonide nasal spray for allergic rhinitis were included to be searched from the date of database construction to March 18, 2023. Meta-analysis was performed using RevMan 5. 4 software after data extraction by 2 investigators, selection of literature, and evaluation of the risk of bias of included articles.Results A total of 16 RCTs including 1277 patients, 638 patients in the study group, and 639 patients in the control group, were included in the study. 0.00001), significant rate (OR = 2.29,95%CI = 1.77 ~ 2.96,P < 0.00001), nasal symptom score (SMD=-1.41,95%CI=-2.30~-0.53,P < 0.00001), interleukin 6 level (MD= -36.06 ,95%CI = − 41.80~ -30.33 ,P < 0.00001 ), interleukin 8 (MD= -24.30,95%CI= -29.71~ -18.89,P < 0.00001) and interleukin 10 (MD = 3.77,95%CI = 3.36 ~ 4.17,P < 0.00001) were significantly The differences were statistically significant. The differences in the incidence of adverse reactions between the study group and the control group were not statistically significant (OR = 0.53,95%CI = 0.16 ~ 1.72, P = 0.29 ). Conclusion: Montelukast sodium combined with budesonide nasal spray is more effective than budesonide nasal spray alone in the treatment of allergic rhinitis.
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