Increased fructose consumption predisposes to nonalcoholic steatohepatitis (NASH).There no effective treatment to be used as the second line when lifestyle modification is insufficient .So, the aim of this study was to evaluate the effect of Curcumin on some biochemical markers in fructose induced NASH . Sixty male albino rats were divided randomly and equally into three groups. Group I (control group). Group II (NASH group), received 70% fructose for 5 weeks .Group III (curcu min treated NASH group), fed as group II follo wed by daily admin istration of curcumin at a dose of 50 mg/ kg orally for 6 weeks. Blood samples were co llected to measure lip id profile, and liver enzy mes activit ies . Liver tissues samples were collected for estimation of mRNA expression levels of nuclear factor E2-related factor-2 (Nrf2) using quantitative real-time PCR ,levels of Beclin 1, AMPK, IL6 and Nrf2 DNA -binding activ ity by ELISA method, and oxidative stress markers malondialdehyde (MDA), supero xide d ismutase (SOD) ,GSH spectrophotometrically. NASH was confirmed by histopatholo gy as well as increased activity of liver en zy mes and marked dyslipidemia .Curcu min ameliorated biochemical disturbance in NASH by upregulating autophagy process through increasing Beclin 1, increasing AMPK and hampered IL6 levels in the liver . It also restored redox balance as evident by suppressing MDA formation and preservation of intracellu lar antio xidants status via increasing GSH, activating SOD and Nrf2 signaling pathway. Results suggested that curcumin has an effective role in counteracting both abnormalities in autophagy process and redox balance, supporting the potential utility of this natural product in effective t reatment of NASH.
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Background: Breast cancer is one of the leading causes of cancer mortality in women worldwide. Despite significant advances in cancer treatment, mortality results from local invasion and/or distant metastasis. Aim: To evaluate EMMPIRIN (CD147) and matrix metalloproteinase-9 (MMP9) immunohistochemical expressions significance in invasive ductal breast carcinoma cases in relation to other clinicopathological parameters. Materials and Methods: The study included 50 specimens of surgically resected breast carcinomas in which the expression of CD147and MMP9 was assessed. The results were compared statistically with chi-square (χ2) and Fisher exact test. Results: CD174 overexpression showed significant association with poorly differentiated tumors, staging, presence of nodal metastasis and high Ki67 expression. There was a significant correlation between high MMP9 expression and pathological variables as tumor staging, positive nodal metastasis, low ER expression and high HER2 expression. Conclusion: The results of this study suggest that high expressions of EMMPRIN and MMP9 correlate to poor prognostic parameters. Targeted therapy might improve the prognosis of patients with CD147 and MMP-9 overexpression.
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