We have previously proposed that the G protein-coupled receptor, GPR87, may be an LPA receptor. This study evaluated whether GPR87 was functionally involved in the LPA response using ME180 cervical carcinoma cells. Our in vitro wound healing assay revealed that LPA-induced cell migration was suppressed by the LPA antagonist Ki16425. RT-PCR analyses indicated that ME180 cells expressed LPA1 receptor and GPR87, both of which are antagonized by Ki16425. The ME180 cells pretreated with gpr87-specific siRNAs significantly reduced LPA-induced cell migration. These results suggest that GPR87 is activated by LPA and the activation of GPR87 leads cell migration.
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