In 42% of the osteosarcomas, the tumor cells expressed ErbB-2. Expression of ErbB-2 was strongly correlated with early pulmonary metastasis and poor survival rate for the patient. These data suggest that ErbB-2 plays a significant role in aggressive tumor growth and in the promotion of metastatic potential in osteosarcomas. ErbB-2 in the osteosarcoma tissues would be a useful prognostic marker for patients.

No abstract

of Japan. 137 Cs) through rivers into the sea have been studied. The solid-water distribution of RCs in river water is important because hydrated RCs, which is not subject to flocculation and sedimentation processes in the estuary area, may be the main form of RCs transported into oceans. Takata et al. (2015) evaluated the flux of dissolved and particulate 137 Cs from the river to the ocean to calculate the migration of 137 Cs in the ocean. The solid-water distribution is also important in terms of the intake of organisms. Thus, the solid-water distribution of RCs has been intensively studied with respect to the emission of RCs associated with the FDNPP accident (

Extra-abdominal desmoid tumours (EADT) are benign lesions but difficult to cure because of their infiltrative nature and tendency to recur. Among many treatments recommended in the past, wide excision has been successful, even in difficult cases. We have analyzed retrospectively 41 cases of histologically confirmed EADTs. A total of 98 operations were performed on these patients: 29 wide excisions on 22 patients, 52 intra-lesional excisions with wide margins on 16 patients, and 17 incomplete excisions on 3 patients. One patient, with intra-pelvic lesions, died of a massive haemorrhage 3 days after surgery. Forty patients were followed from between 3 and 29 years. One, who had a multicentric EADT for 21 years, died from the disease. The significant factors concerning local recurrence after wide procedures were an unsatisfactory initial wide local excision, disease affecting 4 or more muscles and the invasive nature of the recurrences. We recommend wide local excision of these tumours in all anatomical areas that allow this procedure. When major nerves and vessels are involved, we recommend an intralesional excision with wide margins in order to preserve limb function. Radiation therapy should be confined to cases in which wide local procedures are not feasible. Overall, 37 of our patients (90%) were cured of the disease, 2 had their disease controlled, and 2 died.

BACKGROUND.It has been reported that the c-erbB-2 protooncogene is frequently amplified and overexpressed in many types of cancers, except sarcomas and hematological malignancies. METHODS. Expression of ErbB-2 in the tumors of 26 patients with conventiional osteosarcoma was evaluated by immunoblotting. DNA from osteosarcoma tissues that expressed ErbB-2 were analyzed by Southern blot hybridization to examine gross rearrangement of the gene. The DNA was also surveyed for the presence of genetic mutation in the transmembrane domain of ErbB-2 by polymerase chain reaction-single-stranded DNA conformation polymorphism analysis. In addition, possible correlation of ErbB-2 expression with gender, age, histopathologic subtype, and response to chemotherapy was analyzed. Survival analysis was performed by the Kaplan-Meier test using the approximate chi-square statistic for the logrank test. RESULTS. The ErbB-2 protein was detected in 11 of 26 osteosarcoma tissues (42%) by immunoblot analysis. Expression of ErbB-2 was confirmed by imrnunohistochemical studies using specific anti-ErbB-2 monoclonal antibody. However, neither amplification of the c-erbB-2 gene nor evidence of significant genetic mutation was found in these osteosarcomas. Expression of ErbB-2 examined by immunoblot-

Spherical radioactive caesium (cs)-bearing microparticles (csMps) were emitted during the fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident in March, 2011. The emission source (timing) and formation process of these particles remain unclear. In this study, the isotopic ratios of uranium (235 U and 238 U) and caesium (133 Cs, 134 Cs, 135 Cs, and 137 Cs) isotopes in the five spherical CsMPs (ca. 2 μm in size) sampled at 50 km west of the FDNPP were determined using secondary ion mass spectrometry and laser ablation-ICPMS, respectively. Results showed that the 235 U/ 238 U ratios of csMps were homogeneous (1.93 ± 0.03, N = 4) and close to those estimated for the fuel cores in units 2 and 3, and that the Cs isotopic ratios of CsMP were identical to those of units 2 and 3. These results indicated that U and Cs in the spherical CsMPs originated exclusively from the fuel melt in the reactors. Based on a thorough review of literatures related to the detailed atmospheric releases of radionuclides, the flow of plumes from the fDnpp reactor units during the accident and the U and cs isotopic ratio results in this study, we hereby suggest that the spherical CsMPs originate only from the fuel in unit 2 on the night of 14 March to the morning of 15 March. The variation range of the analysed 235 U/ 238 U isotopic ratios for the four spherical particles was extremely narrow. Thus, U may have been homogenised in the source through the formation of fuel melt, which ultimately evaporating and taken into CsMPs in the reactor and was released from the unit 2. On 11 March 2011, the Great East Japan Earthquake triggered the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident. Radioactive materials were released to the atmosphere and have caused various environmental problems 1-4. Among the six nuclear reactors in the FDNPP, the reactors of units 1-3 were in operation at the time of the earthquake 5. In the accident, hydrogen explosions occurred in the nuclear reactor buildings of units 1, 3 and 4 (at 15:36 JST on 12 March, 11:01 JST on 14 March and 6:14 JST on 15 March, respectively), but the explosion in unit 4 occurred in the reverse flow of hydrogen generated in unit 3 6. Unit 4 had been shut down from 2010, and all of the fuel were kept in the spent fuel pool (SFP), suggesting that no radionuclide was emitted from unit 4, whereas units 1 and 3 can be the sources of the radionuclides. Meanwhile, the release of radionuclides from reactor pressure vessel (RPV) or blowout panel in unit 2 was also an important source of the radionuclides 7 .

A part of radiocesium emitted during the fukushima nuclear accident was incorporated in glassy water-resistant microparticles, called Type-A particles, which are spherical with ~ 0.1 to 10 µm diameter and ~ 10-2 to 10 2 Bq cesium-137 (137 Cs) radioactivity; they were emitted from Unit 2 or 3 of the Fukushima Daiichi Nuclear Power Plant. Meanwhile, Type-B particles, having various shapes, 50-400 µm diameter, and 10 1-10 4 Bq 137 Cs radioactivity, were emitted from Unit 1. The chemical properties of these radioactive particles have been reported in detail, but previous studies investigated only a small number of particles, especially Type-B particles. We tried to understand radioactive particles systematically by analyzing a large number of particles. Micro-X-ray computed tomography combined with X-ray fluorescence analysis revealed the presence of many voids and ironrich part within Type-B particles. The 137 Cs concentration (Bq mm-3) of Type-A particles was ~ 10,000 times higher than that of Type-B particles. Among the Type-B particles, the spherical ones had higher concentration of volatile elements than the non-spherical ones. These differences suggested that type-A particles were formed through gas condensation, whereas type-B particles were formed through melt solidification. These findings might contribute to the safe decommissioning of reactors and environmental impact assessment. On March 11, 2011, a great earthquake hit the eastern part of mainland Japan and triggered several gigantic tsunami waves, attacking the six-unit Fukushima Daiichi Nuclear Power Plant (FDNPP). The tsunamis damaged the electric functions to cool reactor cores and rapidly increased the temperature in the primary containment vessels (PCVs) in Units 1-3 that were operating during the accident. In addition, the chemical reactions of water and zirconium for fuel cladding at high temperature increased the pressure inside the reactors due to the large amount of hydrogen (H) gas produced. The Tokyo Electric Power Company (TEPCO) tried to vent H and other gases to decrease the pressure. According to TEPCO 1 , H explosions occurred in Units 1 (15:36 JST, March 12,

The reductive effect of an anti-pollinosis mask against internal exposure from radioactive materials dispersed following the Fukushima Daiichi Nuclear Disaster was investigated. A single mask was worn continuously for 18 h from 15:00 JST on 15 March to 09:00 JST on 16 March 2011 at the Hongo campus of the University of Tokyo, Japan. An adult without a mask was exposed during this time to radiation of 6.1 μSv over ambient background in effective dose and 33 μSv in dose equivalent to the thyroid. Radionuclides were dispersed not only in their gaseous and particulate state but also as components that agglomerate to other aerosols and pollens. Wearing a mask for anti-pollinosis could reduce internal exposure from inhalation.