This paper presents the design, simulation and low-cost fabrication of microfluidic channel for biomedical application. Channel is fabricated using soft lithography technique. Printed Circuit Board (PCB) is used to make the master for the channel. Channel pattern is transferred on PCB plate using toner transfer technique followed by ferric chloride etching. Paper also discusses, the issues involved in PCB based master fabrication and their viable solutions. Glass is used as substrate material and the channel is made of Sylgard 184 Polydimethylsiloxane (PDMS). Channel is interfaced with a syringe pump to observe the fluid flow. To predict the behavior of the channel, FEM simulation is performed using COMSOL Multiphysics 5.2a. There is a good match between the theoretical, simulation and test results. Finally, to test the biocompatibility of the channel, genomic DNA is passed through the channel and gel electrophoresis analysis is performed. Microfluidics deals with the study of devices that can handle the very small amount of liquid down to femtoliter with the help of small channels and reservoirs. The dimensions of microfluidic devices range from ten to hundreds of micrometers 1-5. These devices have promising applications in biological analysis 6-9 chemical analysis 10 , optical communication 11 , cooling of Integrated Circuits (ICs) 12 , and many more. In microfluidics, scaling down the device dimensions to microscale reduces the amount of sample and reagents required to perform the assay, resulting in a huge saving in cost and the reduction in the amount of waste produced. Due to low fabrication cost, it can easily be disposed-off. This also reduces the risk of cross-contamination between the tests. The portable analysis devices that can perform the necessary analytical tests outside the central facility at the remote location or in the vicinity of the patient have a huge demand in the biomedical industry. Performing the assay at the location of patient results in real-time test data, which can help medical experts to intervene timely, and this can improve the clinical outcome of the patient 13-17. Fabrication of low-cost sensors, transducers, and biomedical analysis devices were possible due to advances in Micro Electro Mechanical Systems (MEMS) technology. In this technology there are two popular fabrication approaches, first uses silicon and the second, polymers 18-20. Polymer-based MEMS devices widely used in the biomedical industry due to advantages it offers such as biocompatibility, ease of fabrication with the minimum facility, low-cost, ease of disposal and a minimal volume of sample and reagents requirement 9,21,22. These polymer-based devices are also known as BioMEMS or microfluidic devices 23. The microfluidic analysis chip, which enables the complete biomedical analysis without the external intervention, are called micro Total Analysis Systems (μTAS) and are very useful for the Point-of-Care (POC) applications 15. Microchannels, microvalves, micropumps, and micromixtures are few popular examples ...
