To deal with gas and sand problems in their conversion and rod pump wells an operator company in south Texas started introducing a combined technology of two-stages filtration with a modified poor boy gas separator obtaining excellent results. This paper explains the technology used and shares the information used to design the tools and the results achieved in the first wells completed. The screening process to choose the best technology started trying different technologies for gas and sand control below the rod pump. Different technologies were revised sharing data like sand particle size, pump design, fluid production expected and wellbore configuration to get the best design from different companies. The technical and economic evaluation determined the combined system with two-stages filtration and gas separation was the best technology among all the installations. After the results, same technology was applied to other wells changing the configuration based on the well conditions but maintaining the same principle of operation.
After the installation of this technology in each well, it was clear that there was a substantial increase in production among the wells that was caused for the improvement in the pump cards after the installation. The downhole equipment has been able to handle better gas production and no sand problems have been reported so far. The success of this technology has extended the operational capabilities of the pumps allowing the engineers to operate their wells better. Pump cards before and after the installations are summarized in the presentation to show evidence of the good results obtained. After the wells are converted from ESP to rod pump or when the gas represents an issue in the rod pumped wells, the production engineers are limited in the drawdown and the production they can get out of the wells. We are presenting an alternative for the operators to optimize the production's BHA and overcomsand and gas problems that limit the ability to increase the income of the oil fields.
The bacterial pathogen Mycobacterium tuberculosis (Mtb) successfully evades host innate immune responses to cause tuberculosis (TB) disease. Recent studies showed that myeloid-derived suppressor cells (MDSCs) are recruited to the lung after Mtb infection and harbor Mtb. While MDSC-mediated negative regulation of tumor micro-environments in cancer has been extensively studied, in-depth analyses of the phenotype, functions and transcriptional signatures of MDSCs in TB are lacking. In this study we sought to characterize MDSCs in the lungs of Mtb-infected mice and develop tools for depleting MDSCs as a strategy for host-directed therapy. We determined MDSCs in the aerosol mouse model of TB and in human blood using flow cytometry. MDSCs are recruited into the lung as early as 2 weeks post-infection and expresses immunomodulatory mediators associated with immune suppression such as iNOS, IDO and arginase. We recently reported the development of novel synthetic nanoparticle antibodies (SNAbs) that specifically target and deplete MDSCs via antibody-like mechanisms. We used SNAbs to deplete Mtb-induced MDSCs in vitro and in human PBMCs ex vivo. Interestingly, we found that SNAbs efficiently depleted MDSCs in both models. To test the efficacy of SNAb-mediated depletion of MDSCs in mouse lungs in vivo, we first used a murine lung cancer model and found that intra-tracheal delivery of SNAbs successfully depleted MDSCs in vivo. Ongoing studies include evaluating the ability of SNAbs to deplete lung MDSCs following Mtb infection of mice and to assess the impact on lung T cell functions, pathology and Mtb burden. These studies will establish a platform to investigate MDSCs as a target for host-directed therapies that improve TB treatment efficacy.
Supported by grant from NIH (1R01AI155023-01A1)
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