DNA molecules in a solution can be immobilized and stretched into a highly ordered array on a solid surface containing micropillars by molecular combing technique. However, the mechanism of this process is not well understood. In this study, we demonstrated the generation of DNA nanostrand array with linear, zigzag, and fork-zigzag patterns and the microfluidic processes are modeled based on a deforming body-fitted grid approach. The simulation results provide insights for explaining the stretching, immobilizing, and patterning of DNA molecules observed in the experiments.
The dynamic behavior of droplet formation on micropillar surface during a dewetting process is investigated in this paper via a numerical approach. The simulation results suggest that the formation of droplet is governed by the recoiling of captive wet region on the top surface of micropillar after two bridge-shaped meniscus detachments from neighboring droplets. The numerical simulations also show that the cross-sectional shape and its orientation of micropillar play an important role in determining the flow pattern of the dewetting process, especially the evolution and movement of meniscus across the micropillar before a microdroplet is formed. The dimensionless group, i.e., the Ohnesorge number (Oh), the Capillary number (Ca) and the dimensionless liquid thickness (H), characterizes the dimensionless diameter (d) and formation time (t * d ) of droplet. Both can be expressed as the product of polynomial functions of dimensionless group, and approach to an asymptotic solution for H greater than unity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.