RESUMO ABSTRACTContaminação; Desinfecção; Guta-Percha; Bacillus; Staphylococcus. Contaminati on;Disinfecti on; Gutt a-Percha; Bacillus Staphylococcus. DESCRITORES KEYWORDS Objecti ve:To analyze the contaminati on on the surface of gutt apercha cones from diff erent manufacturers available in the nati onal market and to identi fy the isolated microorganisms. Method: Thirty-four accessory gutt a-percha cones obtained from sealed or violated packages were analyzed. Thirty cones were randomly divided in 6 experimental groups with 5 cones each, according to their manufacturer and origin (sealed or violated packages). Four cones obtained from sealed or violated packages were used as positi ve (contaminated with fresh saliva) and negati ve (immersed in 5.25% sodium hypochlorite soluti on for 1 minute) controls. The microbiological analyses were performed by submerging the cones in tubes containing BrainHeart Infusion (BHI) broth, and then seeding an aliquot of this broth in agar blood. The analysis was based on the turbidity of the medium or growth of colonies in the solid medium. Data were analyzed by the Kruskal-Wallis and chi-square tests at 5% signifi cance level.Results: There was bacterial growth in 2 samples. Staphylococcus and Bacillus genera were found. The results indicate that 6.67% of the gutt a-percha cones from sealed or violated packages were contaminated without stati sti cally signifi cant diff erence between the experimental groups. Conclusion:There is a risk of contaminati on of gutt a-percha cones. Therefore, these materials must be disinfected to ensure the safety necessary for the success of endodonti c treatment.
Reducing the accumulation of microorganisms on an endodontic file during endodontic treatment is important to limit recontamination of the root canal and increase likelihood of successful treatment outcome. Objective: To compare the antimicrobial activity of peracetic acid (PA), isopropyl alcohol and acetone against a range of bacteria and also for disinfection of contaminated endodontic K-files. Material and Methods: Antimicrobial activities of PA, isopropyl alcohol and acetone were compared against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, vancomycin resistant E. faecalis (VRE) and meticillin resistant S. aureus (MRSA), using minimum bactericidal concentration (MBC) and time-kill assays. Test solutions at different exposure times (15 s and 30 s) were assessed for treatment of endodontic files acting as carriers of E. faecalis-contaminated dental debris. Results: All bacteria were susceptible to PA (MBC range 0.25-1%), acetone (MBC range 50-60%) and isopropyl alcohol (30-40%). Using a time-kill assay of the antimicrobials at the determined MBC, all test microorganisms, with the exception of E. faecalis (VRE) 7766 were killed after 15 s exposure. In the case of E. faecalis 7766, viable cells remained detectable after 120 s exposure to acetone. Testing disinfection of endodontic Kfiles, previously coated with dental debris containing E. faecalis, it was found that PA (2%) completely killed E. faecalis after 15 s exposure. However, even after 30 s exposure, isopropyl alcohol (80%) and acetone (80%) had limited disinfecting activity. Conclusion: Extrapolation of these results to clinical practice would suggest that PA would be the most effective agent for trans-operative disinfection of endodontic K-files during treatment of a single patient.
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