PG, NDGA, BHT, and BHA were extracted with acetonitrile from fat or oil, followed by thin layer chromatographic separation and identification. The four antioxidants were distinctly separated from one another by one-dimensional solvent development of the silica gel adsorbent layer. The chromatography required < 1 hour from spotting to visualization with 2,6-dichloroquinone chlorimide. As little as 1 μg of BHT and 0.5 μg of PG, NDGA, and BHA were detected by this method.
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