Ureplasma urealyticum (T-mycoplasma) has been related to male infertility by some observers. To assess further this question 150 couples who presented for fertility had semen and cervical mucus cultures for mycoplasma. Positive cultures were obtained from 69 couples. There were no significant differences in the semen analyses among patients with positive or negative cultures. The presence of mycoplasma in the semen is probably the result of contamination at the time of ejaculation. Routine investigation for the presence of mycoplasma in subfertile men is unjustified. The routine use of broad-spectrum antibiotics in subfertile patients with positive mycoplasma culture is unnecessary.
Traditional knowledge, in vitro studies, and studies using animal models suggest that Tridax procumbens L. exhibits blood glucose-lowering properties and antiinflammatory effects. In this study, we evaluated the blood glucose-lowering effect of T. procumbens supplementation in individuals with type 2 diabetes. An extract (asava) of T. procumbens L. was prepared following Ayurveda guidelines. Chemical and microbial analyses indicated presence of phenolics, flavonoids, and carotenoids, and absence of microbial contamination, aflatoxins, heavy metals, and pesticide residues. A chemical fingerprint of T. procumbens L. asava, developed using Ultra high pressure liquid chromatography/electron spray ionization-mass spectrometry (UPLC/ESI-MS) in negative mode, suggest the presence of several compounds including polyphenols. T. procumbens asava demonstrated strong total antioxidant capacity, Fe reducing potential, Fe chelation, H O scavenging activity, and inhibition of lipid peroxidation. We recruited 20 type 2 diabetic individuals from Kolhapur, India. Participants received 15 mL of T. procumbens asava, twice daily, for 4 weeks, while continuing their prescribed antidiabetic medications. Fasting blood glucose decreased by 11% in men (p < 0.01) and 20% in women (p < 0.05), and post-prandial blood glucose concentrations were lowered by 26% in men (p < 0.001) and 29% in women (p < 0.001) following 4 weeks of asava supplementation. No adverse events or side effects were reported. This is the first clinical study demonstrating a significant blood glucose-lowering effect of T. procumbens asava in type 2 diabetes. Copyright © 2015 John Wiley & Sons, Ltd.
Traditional knowledge and anecdotal evidence suggest that Tridax procumbens Linn may have anti‐inflammatory and blood glucose lowering properties. Recent studies have shown that T.procumbens extract was effective in lowering blood glucose (42‐71% blood glucose reduction at 250‐500 mg/kg body weight) in alloxan‐induced diabetic rats. The goals of this study were to evaluate blood glucose lowering potential of T.procumbens extract in subjects with type 2 diabetes and characterize underlying molecular mechanisms. Twenty individuals with established type 2 diabetes were orally administered (15 ml, twice daily) T.procumbens extract, prepared according to Ayurveda guidelines, for a period of 4 weeks. Fasting blood glucose concentrations were reduced by 11% in men and 20% in women, and post‐prandial blood glucose concentrations were decreased by 26% in men and 29% in women. No adverse effects were reported by the participants. Chemical and microbial analyses of T. procumbens extract demonstrated the presence of phenolics, carotenoids, and flavonoids, and absence of microbial contaminants, aflatoxins, and heavy metals. T.procumbens extract demonstrated strong antioxidant potential and anti‐inflammatory activity as evidenced through Trolox equivalent antioxidant capacity, ferric‐ion reducing potential, hydrogen peroxide scavenging activity, and metal ion chelating effect in vitro, and inhibition of NFκB and IkBα in LPS‐stimulated RAW 264.7 murine macrophages. T.procumbens extract significantly increased AMPK activation and suppressed dexamethasone‐induced PEPCK and G6Pase gene expression with subsequent decrease in hepatic glucose production in H4IIE cells. Our studies indicate that T.procumbens extract exhibits strong antioxidant and anti‐inflammatory properties. Activation of AMPK and suppression of hepatic glucose production may be one mechanism that contributes to the glucose‐lowering property of T. procumbens. Grant Funding Source: Diabetes Action Research and Education Foundation
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