This study aimed to determine the quality of drinking water supplied in different types of food stalls in Jashore Municipality, Bangladesh. A total of 35 water samples were collected from different tea stalls, street side fast food stalls, normal restaurants and well-furnished restaurants. The water quality was evaluated by determining the distinct physical, chemical and biological parameters. The results revealed that the water used in the food stalls and restaurants for drinking purpose was in desired quality in terms of turbidity, electrical conductivity, pH, total dissolved solids, nitrate (NO3−), sulfate (SO42−), phosphate (PO43−), chloride (Cl−), sodium (Na) and potassium (K) concentrations. The values were within the permissible limit proposed by the Bangladesh Bureau of Statistics and the World Health Organization. Concentrations of calcium (Ca) and magnesium (Mg) found in several samples were higher than the World Health Organization standard. Iron (Fe) concentrations were higher than the permissible limit of the World Health Organization. Only 46% exceeded the permissible limit of Bangladesh Bureau Statistics. The threatening result was that the samples were contaminated by fecal coliform, indicating that the people of Jashore Municipality may have a greater chance of being affected by pathogenic bacteria. The drinking water provided in the street side fast food stalls was biologically contaminated. The findings demonstrate that the drinking water used in food stalls and restaurants of Jashore Municipality did not meet up the potable drinking water quality standards and therefore was detrimental to public health.