The prediction of prognosis of hepatocellular carcinoma (HCC) following partial hepatectomy is still an unresolved issue. The aim of this study is to identify the association between miRNA-29s family and the prognosis of patients with HCC in a large Asian cohort. We retrospectively reviewed 122 patients with HCC managed in our institution between 2008 and 2015. The expression of miRNA-29s was detected by real-time polymerase chain reaction (PCR). Prognostic factors were evaluated using Kaplan–Meier curves and Cox proportional hazards models. For the entire cohort of 122 patients, the normalized real-time PCR results showed that miRNA-29s (miR-29a, miR-29b, and miR-29c) were deregulated in tumor tissues as compared with corresponding nontumorous tissue samples. We then performed survival analysis to investigate the prognostic value of miRNA-29s. We found that low miR-29b was associated with a decreasing 5-year overall survival (OS) rate from 70.2% to 39.1% and low miR-29c was associated with a decreasing 5-year OS rate from 53.6% to 23.7%. We further conducted multivariate Cox proportional hazards analysis adding the variable of combined low miR-29b and low miR-29c. The results demonstrated that combined low miR-29b and miR-29c was an independent prognostic factor of patients with HCC. In conclusion, we found that the miRNA-29s were down-regulated in tumor tissues as compared with corresponding nontumorous tissue samples. Combined low miR-29b and miR-29c was an independent prognostic factor of patients with HCC.
Aim: Vitamin K2 and phosphatidylcholine are two common drugs in clinical treatment. Studies carried out in the past several years demonstrated vitamin K2 and phosphatidylcholine could separately inhibit hepatocarcinogenesis. In this study, we sought to investigate the synergy of vitamin K2 and phosphatidylcholine and the potential mechanism. Methods: Multiple assays were performed to evaluate the effect of combination administration in vitro and in vivo. Then microRNA microarray, bioinformatics analysis and western blot were performed to explore the potential mechanism of drug action. Results: In vitro, combined administration of vitamin K 2 and phosphatidylcholine for 72 hours showed significant anti-tumor effect in four HCC cell lines (Hep-3B, Hep-G 2 , Huh-7 and SMMC-7721). In vivo, tumor growth was significantly suppressed in the treated group. According to microRNA microarray and bioinformatics analysis, miR-16 was significantly up-regulated and WNT signaling pathway was strongly correlated with the process of anti-tumor. Then western blot analysis indicated that low-expression of WN-T3A, p-β-catenin and Bcl-2 accorded with the assumption of miR-16's function. Conclusions: At last we inferred, given together, vitamin K 2 and phosphatidylcholine exhibited synergy against hepatocarcinogenesis via miR-16 regulating. However, further study is needed to confirm these regulatory relationships.
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