To investigate the clinical efficacy of autologous bone marrow mesenchymal stem cells transplantation in patients with acute spinal cord injury. The clinical manifestations of these patients are abnormal sensation and motor functions that dominated by damaged nerve segment. A total of 36 patients with acute spinal cord injury were randomly divided into control group (n=18, treated with decompression + internal fixation + conventional medical treatments) and experimental group (n=18, control group's treatment + autologous bone marrow mesenchymal stem cells injection in the subarachnoid space). The total effective rate in the observation group was higher than control group (P=0.045). After 12 months of treatment, the SCIM-III score, ASIA exercise score, Botsford score, and ASIA sensory score were significantly higher than those before treatment in both groups, and the increase in the observation group was more obvious (P<0.05). The time for muscle strength and muscle function recovery and independent walking in the observation group were less than those of the control group (P<0.05). Autologous bone marrow mesenchymal stem cells transplantation can improve the therapeutic effect and nerve function, promote the recovery of motor function, sensory function and spinal cord injury without disorder in humoral immune function.
The aim of this study was assessing the mechanism of HIF-1α gene carried with albumin nanoparticles in regulating the tumor angiogenesis in HPV through induction of PI3K/AKT signal pathway. 40 of Wistar rats were divided into blank set, HIF-1α set, agonist
set and inhibitor set randomly. There were 10 rats in every set. The gene expression of HIF-1α, presentation rate of CD31 and SMA, protein expression of VEGF and TSP-1, and presentation of factors related with PI3K/AKT signal pathway were all studied. HIF-1α gene
carried with albumin nanoparticles was prepared successfully, and the gene expression of HIF-1α in HIF-1α set was highest. The second highest expression was in the inhibitor set, while the lowest was in the blank and agonist sets. There were notably-visible tan particles
in the tumor tissue from the blank, HIF-1α and agonist sets, and they were reduced notably in the inhibitor set. The presentation rates for CD31 and SMA in the tumor tissues from the blank, HIF-1α and agonist sets were highest, while the rate was reduced in the inhibitor
set. The action on VEGF protein expression was similar, and there was positive correlation between abnormal activation of HIF-1α and tumor angiogenesis in HPV. The PI3k/AKT signal transduction pathway was activated by HIF-1α and PI3k and AKI were phosphorylated. The
HIF-1α was prompted to enter into cell nucleus and interact with element with low oxygen effect so as to increase the presentation of VEGF, CD31 and SMA. The angiogenesis in the HPV was also prompted, but the action was eliminated by inhibitor from the PI3K/AKT pathway.
Discal cyst is a rare disease, the pathogenesis is not yet clear and its symptoms are very similar to lumbar disc herniation. Although some cases may regress spontaneously, most cases of lumbar discal cysts are treated surgically. At present, there is no consensus on the treatment of this disease. The authors report the clinical usefulness of the percutaneous endoscopic transforaminal surgery technique in two patients with the lumbar 4-5 discal cyst. The clinical symptoms of both patients were unilateral lower extremity pain and lower back pain. Magnetic resonance imaging of the lumbar spine revealed lumbar discal cysts, causing compression to the spinal dura and roots. Both patients received conservative treatment for more than 6 months, but the clinical symptoms persisted so surgical treatment by percutaneous endoscopic transforaminal surgery without additional discectomy was performed under local anesthesia. The symptoms were relieved immediately after removal of the discal cysts. Postoperative magnetic resonance imaging showed that both patients had complete excision of discal cysts and complete decompression of the treated segmental. There were no recurrent lesions and complications during the follow-up period. We believe that percutaneous transforaminal endoscopic surgery could be a safe, mini-invasive and appropriate method for the treatment of discal cysts.
A new metal–organic framework of [Cd3(4-Hpzc)2(4-pzc)2(DMF)2]n (1, 4-H2 pzc = 4-pyrazolecarboxylic acid, DMF = N, N′d-imethylformamide) was prepared via the solvothermal self-assembly reaction of 4-H2pzc
and Cd(NO3)2 ·4H2O. The porous framework has a photonic band gap of 3.37 eV and has a good photocatalytic activation for methyl violet decomposition under ultraviolet irradiation. After obtaining this fresh compound with a special structure, its application
value for the treatment of endometrial cancer was detected. First, the relative expression of IGFBP-rP1 on intrauterine cells was measured. Second, the activity of the PI3K-AKT signaling route in intrauterine cells was determined.
Background: To assess the efficacy of the superior notch of inferior vertebral pedicle approach of transforaminal percutaneous endoscopic lumbar discectomy for very high-grade inferiorly migrated disc herniation.Methods: Data on 32 consecutive patients operated with percutaneous endoscopic surgery via the superior notch of inferior vertebral pedicle approach were reviewed. Age, gender, clinical diagnoses, operation time, hospitalization time, a self-administered questionnaire composed of the leg pain visual analogue scale (VAS leg pain) and the Oswestry Disability Index (ODI) had been recorded before operation and 1, 3, 6 and 12 months after operation, respectively. The clinical results were assessed at the final follow-up by using modified Macnab criteria. Complications were recorded during follow-up, and postoperative X-ray, CT and MRI examinations were performed.Results: There were 21 males and 11 females. The mean age of patients was 51.8±10.6 years. MRI findings of patients with disc herniation were L2-3 level in 4 case, L3-4 level in 9 cases and L4-5 level in 19 cases, which were correlated with clinical symptoms. All patients completed a 12-month follow-up assessment after surgery. The mean operative duration was 68.2±12.8 min, and hospitalization time was 3.6±0.8 days. At 12 months follow up the VAS leg pain had improved by 6.3 (P< 0.05) and the ODI by 43.6 points (P< 0.05). Twenty-three cases (71.9%) were rated as excellent, 7 (21.9%) as good, 2 (6.2%) as fair at the final follow-up. On the basis of the modified MacNab criteria, the overall excellent and good rate was 93.8%. Two patients (6.2%) had transient dysesthesia after surgery. Postoperative radiological evaluations showed no signs of instability or recurrence of pathology.Conclusion: Transforaminal percutaneous endoscopic lumbar discectomy via the superior notch of inferior vertebral pedicle approach could be a safe and effective minimally invasive technique for very high-grade inferiorly migrated disc herniation. This technique warrants further study and clinical application.
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