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2 volumes PBS + 2%FCS, and centrifuged at 680 × g for 5 minutes. Cells were resuspended in PBS + 2%FCS and filtered through 40 μm nylon mesh. Cells were again centrifuged 680 × g for 5 minutes and treated with anti-CD16/32 antibody for Fc-receptor block (2 μL/mouse) for 5 minutes followed by addition of anti-CD45 magnetic beads (Miltenyi) at a 1/5 volume/volume ratio for 15 minutes. After removing the antibody with two PBS + 2%FCS washes, CD45-positive cells were isolated using Auto-MACS Pro (Miltenyi). Isolated cells were centrifuged once at 340 × g for 5 minutes. and injected into WT mice (3 × 10 6 cells/mouse). Statistical analysis. All values presented here are expressed as means ± SEM, unless noted otherwise. Differences between means were evaluated for significance using ANOVA followed by Fisher'sexact test for multiple comparisons. Differences with a p value < 0.05 were considered statistically significant.

A novel stable organic radical, 2-(5-azaindol-2-yl)-4,4,5,5-tetramethyl-4,5-dihydro-1H-imidazoline-1-oxyl-3-oxide (3), forms a two-dimensional brick-wall architecture based on hydrogen bonding and π-stacking. Low-temperature magnetization curves revealed an S-shaped dependence on the applied field below ca. 3.0 K, which could be interpreted as metamagnetic behavior.

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