An assessment method wherein an aphid colony-infected leaf disk was dipped in an insecticide for 10 s and placed in a plastic zipper bag was developed to easily estimate the number of surviving aphids. This number after 48 h could be estimated by counting the number of honeydew droplets produced by aphids in the bags after 24 h. Although this survey method cannot accurately calculate the mortality rate, it is easy and inexpensive. Therefore, this method is considered to be useful as a simple method for estimating the number of surviving aphids and as a method for easily determining insecticides with reduced effects.
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