An experimental investigation of the foam separation of E . coli from distilled water suspension using a cationic surface-active agent, ethylhexadecyldimethylammonium bromide (EHDA-Br) is presented. Results are evaluated in terms of total cell count, using a membrane filtrat,ion t,echnique. Cell concentrations iri the initial suspensions are varied from 5.0 X lo5 to 1 .0 x 108 cells/ml. Surfactant concentrations in the initial cell suspensions are varied from 0.015 t,o 0.040 mg./ml., and foaming times are varied from 2 to 20 min. The residual quantity of cells decreases exponentially with foaming t,ime to about 0.027c of the initial quantit'y after 20 min. The cell enrichment rat,io, varying from 10 t,o 1,000,000, is an inverse power function of the initial stirfactant concentration and an exponential function of foaming time. Foaminess decreases with increasing initial cell concentrations, and for an initial surfactant concentration of 0.030 mg./ml., ()he residual cell concentration is a 1inea.r function of the initial cell concentration.
An experimental investigation is presented of the foam separation of six species of bacteria: Escherichia coli, Serratia marcescens, Proteus vulgaris, Pseudornonas fEuwrescens, Bacillus cereus, and Bacillus subtilis var niger. A cationic surf actant, ethylhexadecyldimethylammonium bromide is used and results are evaluated in terms of total cell count, using a membrane filtration technique. From similar neutral distilled water suspensions of the pure cultures (approximately 107 cells/ ml.) and using the same operating conditions, ratios of cell concentrations in the residual suspensions to those in the initial suspensions range from 0.0013 for Bacillus subtilis var niger to 0.25 for Serratia marcescens. The presence of bacteria, compared to pure surfactant solutions, produces lower collapsed foam volumes; the foam volumes have a strong influence on the separations achieved with the various species, with enrichment ratios ranging from 27 to 3088 and residual ratios ranging from 0.001 to 0.247.
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