Background: Pancreatic cancer (PC) is a type of malignant gastrointestinal tumor. Long non-coding RNA MCM3AP antisense RNA 1 (MCM3AP-AS1) has been reported to stimulate proliferation, migration and invasion in several types of tumors. However, the role of MCM3AP-AS1 in PC remains unclear.Methods: MCM3AP-AS1, microRNA miR-138-5p (miR-138-5p) and FOXK1 levels were detected using quantitative real time PCR. Cell proliferation, migration and invasion were analyzed. Dual luciferase reporter assay was used to confirm the relationship between MCM3AP-AS1 and miR-138-5p, between miR-138-5p and FOXK1. Protein levels were identified using western blot analysis. Results: MCM3AP-AS1 overexpression promoted proliferation, migration and invasion in PC cells. MCM3AP-AS1 silencing showed a suppressive effect on cell growth in PC cells. Moreover, MCM3AP-AS1 knockdown suppressed tumor growth in mice. Dual luciferase reporter assay demonstrated MCM3AP-AS1 could sponge microRNA-138-5p (miR-138-5p), and FOXK1 could bind with miR-138-5p. Positive correlation between MCM3AP-AS1 and FOXK1 was testified, as well as negative correlation between miR-138-5p and FOXK1. MCM3AP-AS1 promoted FOXK1 expression by targeting miR-138-5p, and MCM3AP-AS1 facilitated growth and invasion in PC cells by FOXK1. Conclusion: MCM3AP-AS1 promoted growth and migration through modulating miR-138-5p/FOXK1 axis in PC, providing insights into MCM3AP-AS1/miR-138-5p/FOXK1 axis as novel candidates for PC therapy from bench to clinic.

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in china, the prevalence of Clonorchis sinensis (C. sinensis) infections is only evaluated at the provincial level by national sampling surveys, and data from villages and counties are still lacking. in this study, we conducted a cross-sectional survey in 10 villages located along the Lalin River in northeast China. Clonorchiasis was diagnosed using a modified Kato-Katz method that detects the C. sinensis egg in stools. A total of 3,068 persons were screened and 2,911 were recruited for the study. Overall, the prevalence of C. sinensis infection was 29.3%. Among 175 participants who were cured after antiparasitic treatment, 54 (30.86%) were re-infected in this survey. After calibration of potential confounders, male gender, occupation as a farmer, smoking, and occasionally or frequently eating raw fish were independent risk factors for C. sinensis infection. the results of laboratory examinations in the C. sinensis/hepatitis B or c virus co-infection group were similar to those in the hepatitis B or c virus mono-infection groups. in conclusion, C. sinensis is highly endemic in villages along the Lalin River, and the primary route of infection is the consumption of raw freshwater fish. Co-infection with C. sinensis did't aggravate the clinical manifestations of viral hepatitis in this cross-sectional study. Clonorchis sinensis (C. sinensis) is an important food-borne zoonotic parasite that has infected approximately 15 million people worldwide; countries in eastern and Southeast Asia, including China, Japan, Korea, and Vietnam, account for a large proportion of infections 1-4. There are 13 million people infected with Clonorchis sinensis in China, which is the country with the largest number of infections in the world 5,6. Three large-scale clonorchiasis investigations have been conducted in mainland China. The first national parasite investigation, which covered 30 provinces/municipalities/autonomous regions (P/M/As) from 1988 to 1992 (hereinafter referred to as 1992), showed that the prevalence of clonorchiasis was 0.37% 7. The prevalence increased to 0.58% in the second national parasite investigation, which included 31 P/M/As from 2001 to 2004 8. Another special clonorchiasis investigation in 27 endemic P/M/As was conducted during the years 2001-2004 and showed that the prevalence was 2.40%, with 12.49 million people infected 8,9. Jilin Province is one of the major endemic regions in China 8. The prevalence of Clonorchis sinensis infections is only evaluated at the provincial level by national sampling surveys in China, and data from villages and counties are still lacking. The current study results supplement C. sinensis infection data in rural areas along rivers in Jilin Province. Persistent and chronic infections of C. sinensis often cause the development and progression of hepatobiliary diseases, such as cholangitis, cholelithiasis, cholecystitis, pancreatitis, hepatic fibrosis, liver cancer, and cholangiocarcinoma (CCA) 10. Cholangiocarcinoma is the most severe complication of C. sinensis infection 11,12 .

Rationale:Congenital hepatic fibrosis (CHF) is an autosomal recessive disease characterized by periportal fibrosis, portal hypertension, and renal cystic disease. Essentially, CHF is a variant of fibrocystic disorder in which liver and kidney are commonly affected. Other frequently associated conditions include Caroli syndrome and polycystic kidney disease. CHF is also a known accompaniment in an array of inherited disorders with multiorgan involvement.Patient concerns:The 20-year-old male patient with declining vision (14 years duration), intermittent gingival bleeding (7 years duration), and abdominal distension (5 years duration), presented with exacerbation of these symptoms during the prior 2 months. The patient had been previously diagnosed with retinal macular degeneration, idiopathic thrombocytopenic purpura, and hepatosplenomegaly.Diagnoses:Liver biopsy showed disordered hepatic acini and fibrous parenchymal banding, indicative of CHF.Interventions:After the treatment of diuresis and liver protectants, the clinical symptoms of the patients were improved. We subsequently recommend chromosomal analysis, although the family refused.Outcomes:Three months after discharge, the patient was followed up by telephone. The patient had obvious abdominal distension and we advised that he should be admitted again. But the family refused.Lessons:CHF is an AR disease resulting in portal hypertension and often associated with renal malformations. CHF is also linked to a number of other disorders, many of which are ciliopathies. Because the clinical manifestations of CHF are nonspecific or lacking, its diagnosis is problematic, relying largely on liver biopsy. Once CHF is identified, physicians are obligated to investigate other organ systems, particularly a search for neuromuscular, retina or renal involvement. This case underscores the value of radiologic imaging, pathologic examination, and genetic testing in successfully diagnosing a rare disease.

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