The effect of pinealectomy on the synthesis of the common alpha-subunit of glycoprotein hormones by the specific secretory cells of the pars tuberalis (PT-specific cells) was examined in male chicks. Expression of melatonin receptor (Mel(1c)) mRNA was demonstrated in the chick pars tuberalis by reverse transcription-polymerase chain reaction analysis. Northern blot analyses revealed that, after pinealectomy, common alpha-subunit mRNA levels were increased in the pars tuberalis of chicks kept under normal lighting (L:D=12 h:12 h), indicating that melatonin inhibits the synthesis of the alpha-subunit of PT-specific cells. Furthermore, alpha-subunit mRNA levels in the pars tuberalis were shown to display similar photoperiod-dependent changes in pinealectomized chicks as in intact animals. Levels of alpha-subunit mRNA in the pars tuberalis were decreased in both pinealectomized and control chicks kept under continuous light (L:D=24 h:0), whereas the levels were enhanced in pinealectomized chicks kept under extended darkness (L:D=1 h:23 h) and under normal lighting. Thus, pinealectomy did not affect the inhibition or stimulation of the alpha-subunit synthesis in the chicken pars tuberalis elicited by continuous light or extended darkness, respectively. Quantitative electron-microscopic analyses showed that, after exposure to continuous light for 30 days, many PT-specific cells were filled with enlarged secretory granules in both pinealectomized and control chicks. Exposure to extended darkness for 30 days caused an increase in the cytoplasmic and nuclear areas of the PT-specific cells. Secretory granules were however larger in pinealectomized than in intact control chicks. These results suggest that the activity of PT-specific cells is mainly regulated by photoperiod.
The possibility of activated charcoal interrupting the enteroenteric circulation of phenobarbital was conducted in rabbits prepared by colectomy biliary drainage to block enterohepatic circulation. Fifty minutes after the administration of phenobarbital IV over ten minutes, activated charcoal (N = 7) or non-adsorbent gel (N = 8) were placed into the intestine at a dose of 4 g/kg. Blood was taken hourly for 5 h from the femoral artery and portal vein for the determination of phenobarbital concentration by the homogeneous enzyme immunoassay. The arterio-portal differences of phenobarbital concentrations were significantly greater in the animals treated with the charcoal at 2, 3 and 4 h after the treatment. There were significantly shorter plasma half lives of phenobarbital in the animals given charcoal (3.8 +/- 0.3 h vs 6.9 +/- 0.9 h, p < .02). This study provided evidence of significant enteroenteric circulation of phenobarbital which can be interrupted by the activated charcoal and removed by the mechanism of intestinal dialysis.
Quantitative information is needed on the directly depolarized area (DDA) induced by high-output energy during a precise mapping procedure to detect the origin of a tachycardia. In the present study, a DDA caused by high-output energy was quantitatively evaluated in the exposed canine heart. In 8 dogs, the right atrial and ventricular surfaces were exposed through a right thoracotomy and pacing with various outputs was delivered from the epicardial surface. A comb-shaped 16 polar electrode array and/or a 224 polar mat electrode array were used for recording the epicardial electrograms. The local activation time was measured at each electrode site, and the relationship of the distance between the electrode location from the pacing site and the local activation time was plotted and fitted to a primary regression line. The intercept of the regression line on the horizontal axis was defined as the radius of the 'DDA' and this was evaluated at each pacing output. The radius of the DDA was 0.6+/-0.1 mm with a 2 V and 3.8+/-0.2 mm with a 10 V output when it was evaluated in a direction perpendicular to the fiber orientation of the pectinate muscle, 0.8+/-0.1 mm with a 2 V and 4.1+/-0.3 mm with a 10 V output in a direction parallel to the pectinate muscle fiber orientation, and 0.9+/-0.3 mm with a 2 V and 3.6+/-0.5 mm with a 10 V output in the right ventricle. The DDA extended according to the increase in stimulation outputs at all sites, and there was no significant difference in the pacing site or the direction of the stimulation propagation. The DDA caused by high-output energy is a purely physical phenomenon that depends only on stimulation output and tissue resistance. The diameter of the DDA exceeded 4 mm (ie, the size of a standard tip electrode for catheter ablation) when pacing was delivered with an output greater than 6 V.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.