Summary1. Reproductive interference is a negative interspecific sexual interaction that adversely affects the fitness of males and females during reproductive process. Theoretical studies suggest that because reproductive interference is characterized by positive frequency dependence it is far more likely to cause species exclusion than the density dependence of resource competition. However, the respective contributions of resource competition and reproductive interference to species exclusion, which have been frequently observed in many competition studies, remain unclear. 2. We show that reproductive interference is a far more critical cause of species exclusion than resource competition in the competition between Callosobruchus bean weevil species. In competition experiments over several generations, we manipulated the initial relative abundance of the adzuki bean beetle, Callosobruchus chinensis, and the southern cowpea beetle, Callosobruchus maculatus. When the initial adult ratio of C. chinensis : C. maculatus were 6 : 2 and 4 : 4, C. chinensis excluded C. maculatus. However, when C. maculatus was four times more abundant than C. chinensis at the start, we observed the opposite outcome. 3. A behavioural experiment using adults of the two species revealed asymmetric reproductive interference. The fecundity and longevity of C. maculatus females, but not those of C. chinensis females, decreased when the females were kept with heterospecific males. Fecundities of females of both species decreased as the number of heterospecific males increased. In contrast, resource competition at the larval stage resulted in higher survival of C. maculatus than of C. chinensis. 4. These results suggest that the positive frequency-dependent effect of reproductive interference resulted in species exclusion, depending on the initial population ratio of the two species, and the asymmetry of the interference resulted in C. chinensis being dominant in this study, as in previous studies. Classical competition studies should be reviewed in light of this evidence for reproductive interference.

Reproductive interference is any interspecific sexual interaction that reduces the reproductive success of females through promiscuous reproductive activities of heterospecific individuals. This phenomenon is ubiquitous in nature in both plants and animals, and is frequently observed in biological invasions. However, its effects on interspecific competition remain incompletely understood despite growing concern. To study the interactive effects of resource competition and reproductive interference on species coexistence and exclusion, we analyzed a unified competition model including both processes in symmetric and asymmetric scenarios. The results of our model showed that resource competition and reproductive interference act synergistically to promote competitive exclusion. We also found that when the two processes are asymmetric, the species that is superior in reproductive interference can coexist with or even exclude the species that is superior in resource competition. Therefore, coexistence is possible via an unbalanced trade-off between resource use and reproduction. Our results suggest that integration of reproductive interference and resource competition will contribute to a better understanding of interspecific competition and to more effective biodiversity conservation against management of biological invasions.

Many studies that have researched interspecific competition in Callosobruchus (bean beetles), Drosophila (fruit flies), and Tribolium (flour beetles) have considered the major drivers of interspecific competition to be interspecific resource competition and intraguild cannibalism. These competition drivers have a density‐dependent effect on the population dynamics. However, some studies have also detected a relative‐frequency‐dependent effect in the observed population dynamics. The most likely causal mechanism of this relative frequency dependence is reproductive interference, defined as any interspecific sexual interaction that damages female reproductive success. Reproductive interference has been overlooked by most laboratory studies in spite of the critical effect on the competition outcome. In this paper, I review laboratory studies of these insect genera from the perspective of reproductive interference and show that the reported results can be more reasonably interpreted by the joint action of reproductive interference and resource competition, including intraguild cannibalism. In addition, on the basis of results reported by a small number of related studies, I discuss the behavioral and evolutionary changes induced in those genera by reproductive interference.

Identifying behavioural basis of competitive relationship is essential to understand outcome of interspecific competition. However, it remains difficult to investigate demographic effect of competitive behaviour, because various kinds of behaviours may co-occur in the competition and make the dynamics far complicated in nonlinear ways. We report that the behavioural basis of interspecific interaction can be identified, by focusing on the timescale difference from the occurrence of each behaviour to the appearance of its demographic effect. Between two bean beetles, Callosobruchus chinensis and C. maculatus, major interspecific interactions are resource competition (RC) at the larval stage and reproductive interference (RI) at the adult stage. RC has longer time lag than RI, because effect of RC appears in the adult number of the next generation through larval competition while effect of RI appears instantaneously in the adult number through early death of females. If we detect two effects with different time lags from the competition dynamics, an effect with intergenerational time lag and with no time lag would be considered as RC and RI, respectively. We applied empirical dynamic modelling approach, which is a nonlinear time series analysis for detecting causal interactions and the strength, to two published datasets of experimental competition between those beetles. Results showed the significant causality from the winner species to the loser one in both experiments, but the causality time lag differed between experiments: the causality had no time lag in the C. chinensis-win data, while intergenerational time lag in C. maculatus-win data. Furthermore, detection of the causality with intergenerational time lag from C. maculatus to C. chinensis in both experiments suggests interplay of constant RC and variable RI which can reverse the outcome. This study is the first successful case study that links behavioural-level interactions to demographic-level effects in interspecific competition.

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