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In this study, we investigated the bacterial diversity of Moutai Daqu, Zhongxing Daqu and Zhengjiu Daqu from Guizhou Province of China by high-throughput sequencing method. Sequencing results showed that 35 bacterial families were detected in the three Daqu samples. More interestingly, Sporolactobacillaceae, Streptomycetaceae, Pseudomonadaceae, Actinopolysporaceae, Caulobacteraceae, Pseudonocardiaceae, Nocardiaceae and Methlobacteriaceae were reported foe the first time in Moutaiflavour Daqu using high-throughput sequencing method. In particular, Thermoactinomycetaceae and Bacillaceae were demonstrated to be the common dominant bacteria in the three samples. These new data offer a more complete view of comprehensive of high-throughput sequencing method to understand bacteria diversity of Moutai-flavour Daqu.

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The objective of this study was to isolate and screen the mould microbiome of five Moutai‐flavor Daqu samples and to characterize and quantify the associated exogenous enzymes. In all, 11 and 18 mould genera were identified in the samples by, respectively, a culture‐dependent method and a culture‐independent high throughput sequencing method. Species of Aspergillus and Rhizopus were the dominant mould genera in the Moutai‐flavor Daqu samples. Analysis of enzyme production showed that Aspergillus versicolor in Daqu ZX01 exhibited the highest neutral proteinase activity (2671.0 ± 73.9 U/g) and Rhizomucor pusillus in Daqu DYT01 exhibited the highest saccharifying amylase production ability (1724.6 ± 11.0 U/g). Copyright © 2017 The Institute of Brewing & Distilling

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