Background: Little knowledge about long non-coding RNAs(lncRNAs) in nasopharyngeal carcinoma (NPC) has been acquired.Methods: Next-generation sequencing was applied in 7 cases of NPC tissues and 7 cases of normal tissues in nasopharynx. PLEX, CNCI and CPAT soft-wares were used to predict novel lncRNAs. Real-time Quantitative PCR (qPCR) further validated the data in 20 cases of NPC tissues and 14 cases of normal tissues. Then the cis-regulators and trans-regulators and potential biological functions together with pathways were predicted by Bioinformatics.Results: Totally, 4248 novel lncRNAs were found to be expressed in our samples. And 2192 lncRNAs and 23342 mRNAs were considered to be differentially expressed in NPC. Among the results, 306 lncRNAs and 4599 mRNAs were significantly up-regulated, whereas 204 lncRNAs and 2059 mRNAs were significantly down-regulated, respectively. Moreover, 62 lncRNAs trans-regulated genes were involved in Epstein-Barr virus (EBV) infection pathway in our study. Jun proto-oncogene (JUN), which was related to a cis-regulator lncRNA RP4-794H19.1, was enriched in cancers and involved in Tumor Necrosis Factor (TNF) signaling pathway, might play a key role in NPC.Conclusion: These findings broadened the lncRNAs landscape of NPC tissues and shed light on the roles of these lncRNAs, which might be conducive to the comprehensive management of NPC.

Abstract. The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in the majority of tumor cells, whilst sparing normal cells. However, the potential use of TRAIL in the treatment of cancer is limited by the inevitable emergence of drug resistance. The present study reports the upregulation of latent membrane protein 1 (LMP1)-induced TRAIL resistance via the enhanced expression of X-linked inhibitor of apoptosis protein (XIAP) in nasopharyngeal carcinoma (NPC) cells. LMP1-positive NPC cells were indicated to be more sensitive to TRAIL compared with LMP1-negative NPC cells in three NPC cell lines. CNE-1 is a LMP1-negative NPC cell line that was transfected with pGL6-LMP1; following which, sensitivity to TRAIL decreased. LMP1-induced TRAIL resistance was associated with the decreased cleavage of caspase-8,-3 and -9, BH3 interacting domain death agonist (Bid) and mitochondrial depolarization, without any effects on the expression of the death receptors, B-cell lymphoma (Bcl)-2 and Bcl-extra long. Knockdown of XIAP with small interfering RNA increased caspase-3 and -9 and Bid cleavage, and prevented LMP1-induced TRAIL resistance. Furthermore, embelin, the inhibitor of XIAP, prevented LMP1-induced TRAIL resistance in the Epstein-Barr virus (EBV)-positive CNE-1-LMP1 and C666-1 NPC cell lines. However, embelin did not enhance TRAIL-induced apoptosis in NP-69, which was used as a benign nasopharyngeal epithelial cell line. These data show that LMP1 inhibits TRAIL-mediated apoptosis by upregulation of XIAP. Embelin may be used in an efficacious and safe manner to prevent LMP1-induced TRAIL resistance. The present study may have implications for the development and validation of novel strategies to prevent TRAIL resistance in EBV-positive NPC.

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【摘要】目的：观察电针治疗急性腰扭伤的临床疗效以及治疗前后腰部红外热像图的变 化。方法: 采用随机数字表将 295 例急性腰扭伤患者随机分为电针组 147 例和药物组 148 例，电针组选用后溪穴治疗，药物组用美洛昔康片治疗，各组治疗前后进行腰部红外热 像图的检查。结果: 电针组和药物组痊愈率分别为 71.4%和 42.6%；有效率分别为 93.9% 和 87.2% (P<0.01)；电针组与药物组红外热像图治疗前后温度差分别为为 2.52℃和 0.80℃ (P<0.01)，二者有显著性差异。结论: 电针组和药物组治疗急性腰扭伤均有显著疗效，但 电针组的总体疗效优于药物组，且电针组治疗前后红外热像图的升温效应也明显高于药 物组。 【关键词】腰痛；扭伤和劳损；电针；针刺疗法 【Abstract】 Objective:To observe the clinical effect and infra-red thermogram changes for acute lumbar sprain. Methods: All 295 cases with acute lumbar sprain were randomly divided into an electroacupuncture group (147 cases) and medication group (148 cases). The cases in electroacupuncture group were treated by needling Houxi (SI 3), whereas those in the medication group were treated with Meloxicam tablet. The infra-red thermogram was observed before and after treatment. Results: The recovery and effective rates in the electroacupuncture group were 71.4% and 93.9%, whereas the rates in the medication groups were 42.6% and 87.2% (P<0.01). The temperature differences via the thermogram in the electroacupuncture and medication groups were 2.52℃ and 0.80℃ (P<0.01), indicating a significant difference. Conclusion: Both electroacupuncture and medication could obtain significant effect for acute lumbar sprain; however, electroacupuncture obtained a better overall effect and a more substantial temperature-raising effect in the thermogram than medication. 【Key Words】 Low Back Pain; Sprain and Strains; Electroacupuncture; Acupuncture Therapy 【CLC Number】R246.2 【Document Code】A Acute lumbar sprain is mainly characterized by lower back pain with restricted movement due to uncoordinated contraction of the lumbar muscles and subsequent muscle, fascial tearing or lumbar vertebral joint strain [1] . Literature reports have shown that acupuncture can obtain exact effects for acute lumbar sprain. Over the past two years, we have employed multi-centered, large sample, randomized clinical trials to observe the effect and thermogram changes on electroacupuncture for acute lumbar sprain. The result is now summarized as follows.

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【摘要】目的：观察针刺结合药敷治疗增生性膝骨关节炎的疗效。方法：将 328 例增生 性膝骨关节炎患者，随机分入观察组或对照组，每组 164 例。观察组采用针刺结合药敷 治疗，对照组口服追风透骨丸治疗，治疗 40 天后比较两组临床疗效。结果：两组显效率 及有效率均有统计学差异(P<0.05) 。结论：针刺结合药敷治疗增生性膝骨关节炎疗效优 于单纯口服追风透骨丸。 【关键词】骨关节炎，膝；针刺疗法；针药并用 【Abstract】Objective: To observe the effect of treatment for proliferative knee osteoarthritis (KOA) using combined acupuncture and medicated compression. Method: All 328 cases were randomized into an observation and control group, 164 cases in each. Combined acupuncture and medicated compression were adopted in the observation group, while oral administration of Zhui Feng Tou Gu Wan (Wind-removing and Bone-penetrating Pills) were adopted in the control group. The clinical effects in the two groups were evaluated 40 days after the treatment. Result: There were significant differences in both marked and total effective rates (P<0.05). Conclusion: Combined acupuncture and medicated compression can obtain better effect for KOA than oral administration of the Wind-Removing and Bone-Penetrating pills. 【Key Words】Osteoarthritis, Knee; Acupuncture Therapy; Acupuncture Medication Combined 【CLC Number】R246.2 【Document Code】A Proliferative knee osteoarthritis (KOA) results from bone growth and often occurs in the middleaged or senile patients. In mild cases, patients may experience soreness, pain or discomfort of the knee joint and difficulty walking. In severe cases, patients may experience intolerable pain with an inability to walk. The author treated 164 KOA cases using combined acupuncture and medicated compression. The result is now reported as follows.

In angiosperms, the first zygotic division usually gives rise to two daughter cells with distinct morphologies and developmental fates, which is critical for embryo pattern formation; however, it is still unclear when and how these distinct cell fates are specified, and whether the cell specification is related to cytoplasmic localization or polarity. Here, we demonstrated that when isolated from both maternal tissues and the apical cell, a single basal cell could only develop into a typical suspensor, but never into an embryo in vitro. Morphological, cytological and gene expression analyses confirmed that the resulting suspensor in vitro is highly similar to its undisturbed in vivo counterpart. We also demonstrated that the isolated apical cell could develop into a small globular embryo, both in vivo and in vitro, after artificial dysfunction of the basal cell; however, these growing apical cell lineages could never generate a new suspensor. These findings suggest that the initial round of cell fate specification occurs at the two-celled proembryo stage, and that the basal cell lineage is autonomously specified towards the suspensor, implying a polar distribution of cytoplasmic contents in the zygote. The cell fate transition of the basal cell lineage to the embryo in vivo is actually a conditional cell specification process, depending on the developmental signals from both the apical cell lineage and maternal tissues connected to the basal cell lineage.

The genome sequences of numerous organisms are available now, but gene sequences alone do not provide sufficient information to accurately deduce protein functions. Protein function is largely dependent on the association of multiple polypeptide chains into large structures with interacting subunits that regulate and support each other. Therefore, the mapping of protein interaction networks in a physiological context is conducive to deciphering protein functions, including those of hypothetical proteins. Although several high-throughput methods to globally identify protein interactions have been reported in recent years, these approaches often have a high rate of nonspecific or artificial interactions detected. For instance, the fraction of false positives of the protein interactions identified by yeast two-hybrid assay has been predicted to be of the order of 50%. We have developed a strategy to globally map Bacillus subtilis protein-protein interactions in a physiological context by fractionating the cell lysates using size-exclusion chromatography (SEC), followed by proteome analysis. Components of both known and unknown protein complexes, multisubunits and multiproteins, have been identified using this strategy. In one case, the partners of the B. subtilis protein complex have been coexpressed in Escherichia coli, and the formation of the overexpressed protein complex has been further confirmed by a pull-down assay.