This study investigated whether pretreatment with puerarin could alleviate myocardial ischemia/reperfusion (I/R) injury in a cardiomyocyte oxygen-glucose deprivation and reoxygenation (OGD/R) model and in a mouse I/R injury model. For in vitro experiments, H9C2 cells were divided into control, erastin, OGD/R, OGD/R puerarin, and OGD/R ferrostatin (Fer)-1 groups. Parameters related to ferroptosis included levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), ATP, reactive oxygen species (ROS), glutathione (GSH), prostaglandin endoperoxide synthase (Ptgs) 2 mRNA, glutathione peroxidase (GPX) 4 protein and iron. In H9C2 cells, puerarin or Fer-1 pretreatment reduced ferroptosis, as indicated by decreased ROS and increased GSH, ATP levels. In vivo, wild-type mice were randomly divided into sham, I/R vehicle, I/R puerarin, and IR Fer-1 groups. The I/R model was established by 30 min of left anterior descending artery occlusion followed by 24 h of reperfusion. Pretreatment with puerarin or Fer-1 significantly reduced infarct size in I/R mice, and decreased the activities of Myeloperoxidase (MPO) and cardiac enzymes such as creatine kinase MB isoenzyme (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) compared to those in the vehicle-treated group. Puerarin also reduced the production of MDA and 4-HNE, reduced the mRNA expression of Ptgs2 mRNA, and increased GPX4 protein expression. These results showed that puerarin exerted protective effects against myocardial I/R injury by inhibiting ferroptosis and inflammation, and therefore may have therapeutic potential for treatment of acute myocardial infarction.
To effectively reduce friction torque and start-up wind speed, magnetic bearing was applied to the small-scale wind turbine. Load analysis is the primary task of the design for magnetic bearing in wind turbine. Based on a typical blade model, the aerodynamic performance of the impeller was simulated using Computational Fluid Dynamics (CFD) method. The characteristics and differences of the impeller’s aerodynamic performance under different wind speeds were analyzed and the aerodynamic loads were calculated as well. Moreover, the bearing capacity of magnetic bearing was calculated according to the forces on the spindle. A kind of permanent magnetic bearing (PMB) was designed for the radial supporting of the spindle in wind turbine. A prototype of the small-scale maglev wind turbine (SMWT) was presented, which provides some basis for the application of magnetic bearings in wind turbines.
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