In order to investigate the anti-aging effects of Lycium ruthenicum Murr. granules (HGQ) and potential underlying mechanism of actions, Caenorhabditis elegans was exploited as model organisms. The anti-aging capacity of HGQ was determined by lifespan and lipofuscin fluorescence assays in wildtype C. elegans N2. Calorie restriction pathway was detected by pharyngeal pumping rate assay and lifespan examination in sir-2.1 mutant nematodes. The antioxidant activity of HGQ was investigated by determining reactive oxygen species (ROS) level, paraquat-stress survival rate, malondialdehyde (MDA) content as well as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities. We observed that HGQ exerted little effect on the growth of wild-type N2 nematodes at concentration < 0.6 mg/ml, and 0.05 mg/ml of the HGQ significantly prolonged the lifespan and reduced the lipofuscin level in N2 nematodes. However, the HGQ failed to influence the pharyngeal pumping rate in N2 nematodes or to affect the lifespan of sir-2.1 mutant nematode VC199, indicating the anti-aging effect of HGQ is independent of calorie restriction pathway. Further experiments showed that the HGQ decreased ROS and MDA contents and increased the survival rate and SOD (not CAT or GPx) activities in paraquat-stressed N2 nematodes. Our results suggest that HGQ can effectively delay the senescence of C. elegans, and this effect may be related with its significant antioxidant activity.
A glassy carbon electrode (GCE) was modified with silver-diethyldithiocarbamate (DDTC Ag) modified electrode has been constructed. The DDTC Ag modified electrode showed an excellent electrocatalytical effect on the oxidation of dopamine. In the pH 2.56 buffer solution, the oxidation peak of dopamine was linear with its concentration from 5.0 × 10 -7 -6.0 × 10 -5 mol L -1 , detect limit of it reached 1.0 × 10 -7 mol L -1 . Moreover, the interference of ascorbic acid with the determination of dopamine could be eliminated. The method could obtain better result when it applied to the determination dopamine in serum and medical samples.
A simple flow injection chemiluminescence method was developed for the rapid and sensitive determination of sarafloxacin in animal eggs. The study based on the chemiluminescence induced by H2O2 and luminol in NaOH medium, sarafloxacin could dramatically enhance chemiluminescence intensities and incorporated with solid-phase extraction. The chemiluminescence system has been applied for the determination of sarafloxacin in eggs. Under optimum conditions, the chemiluminescence intensities were linearly related to the concentration of sarafloxacin in the range of 2.0 × 10-7 to 8.0 × 10-5 g L-1 with a correlation coefficient of 0.9992 and the detection limit was 4.0 × 10-8 g L-1. The relative standard deviation was 2.4 % for 5.0 × 10-6 g L-1 sarafloxacin.
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