Ethical and biosafety aspects of this study were performed in accordance with Resolution n°196/96 of National Board of Health/National Research Ethics Committee and were approved by Ethics Committee on Animal Use (CEUA) of PUCPR, statement n°719. 2.1 Animals, experimental conditions and sampling Forty-eight hours before transportation, feeding (commercial diet containing 32% crude protein, SUPRA, Alisul Alimentos,
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